Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil
Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil
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DOI：
10.1089/ees.2013.0067
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发表时间：
2013-11-01
影响因子：
1.8
通讯作者：
Aitken, Michael D.
中科院分区：
文献类型：
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作者：
Dunlevy, Sage R.;Singleton, David R.;Aitken, Michael D.
稳定同位素探测以前被用来确定细菌蒽降解剂在未经处理的土壤从以前的天然气工厂网站。然而，随后的pyrosequence分析的总细菌群落和16S rRNA基因的定量表明，相对丰度的主要蒽降解细菌（指定蒽组1）减少的结果，在实验室规模的生物处理条件下，好氧生物反应器。本研究确定了生物反应器处理的土壤中的Alphaproteobacterial蒽降解细菌，这些细菌与先前确定的细菌不同。最大的序列组来自Alterythrobacter属，而其他序列组与根瘤菌目和慢生根瘤菌属内的细菌相关。生物反应器中的条件富集了能够降解蒽的生物体，这些生物体与未处理土壤中鉴定为主要降解物的生物体不同。此外，这些数据表明，在受污染但未经处理的土壤中鉴定多环芳烃降解细菌可能是生物处理过程中最活跃的降解剂的不良指标。
Stable-isotope probing was previously used to identify bacterial anthracene-degraders in untreated soil from a former manufactured gas plant site. However, subsequent pyrosequence analyses of total bacterial communities and quantification of 16S rRNA genes indicated that relative abundances of the predominant anthracene-degrading bacteria (designated Anthracene Group 1) diminished as a result of biological treatment conditions in lab-scale, aerobic bioreactors. This study identified Alphaproteobacterial anthracene-degrading bacteria in bioreactor-treated soil which were dissimilar to those previously identified. The largest group of sequences was from the Alterythrobacter genus while other groups of sequences were associated with bacteria within the order Rhizobiales and the genus Bradyrhizobium. Conditions in the bioreactor enriched for organisms capable of degrading anthracene which were not the same as those identified as dominant degraders in the untreated soil. Further, these data suggest that identification of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated but untreated soil may be a poor indicator of the most active degraders during biological treatment.