Methods for Studying Mouse and Human Invariant Natural Killer T Cells.
Methods for Studying Mouse and Human Invariant Natural Killer T Cells.
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研究小鼠和人类不变自然杀伤 T 细胞的方法。
DOI：
10.1007/978-1-0716-1775-5_4
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yang,Lili
中科院分区：
文献类型：
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作者：
Zhou,Yang;Li,Yan-Ruide;Zeng,Samuel;Yang,Lili
不变性自然杀伤T细胞（iNKT）是T淋巴细胞的一个独特亚群，可识别由非多态性主要组织相容性复合体（MHC） i样分子CD1d呈现的脂质抗原。iNKT细胞在调节对抗癌症、病毒感染、自身免疫性疾病和过敏的免疫反应中发挥重要作用。然而，iNKT细胞的研究和应用一直受到其数量非常少（小鼠和人血液中的0.01-1%）的阻碍。在这里，我们描述了以下方案：(1)从小鼠单个核细胞或用iNKT T细胞受体（TCR）基因改造的小鼠造血干细胞（分别称为mMNC-iNKT细胞或mHSC-iNKT细胞）中生成小鼠iNKT细胞；(2)从人外周血单个核细胞或用iNKT TCR基因改造的人HSC细胞（分别称为hpmc -iNKT细胞或hHSC-iNKT细胞）中生成人iNKT细胞；(3)在体外和体内对小鼠和人iNKT细胞进行表征。
Invariant natural killer T (iNKT) cells are a unique subset of T lymphocytes that recognize lipid antigens presented by nonpolymorphic major histocompatibility complex (MHC) I-like molecule CD1d. iNKT cells play essential roles in regulating immune responses against cancer, viral infection, autoimmune disease, and allergy. However, the study and application of iNKT cells have been hampered by their very small numbers (0.01–1% in mouse and human blood). Here, we describe protocols to (1) generate mouse iNKT cells from mouse mononuclear cells or from mouse hematopoietic stem cells engineered with iNKT T cell receptor (TCR) gene (denoted as mMNC-iNKT cells or mHSC-iNKT cells, respectively), (2) generate human iNKT cells from human peripheral blood mononuclear cells or from human HSC cells engineered with iNKT TCR gene (denoted as hPBMC-iNKT cells or hHSC-iNKT cells, respectively), and (3) characterize mouse and human iNKT cells in vitro and in vivo.