Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA sequences

Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA sequences
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DOI:
10.1073/pnas.93.17.9188
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发表时间:
1996-08-20
影响因子:
11.1
通讯作者:
Pace, NR
Pace, NR
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Barns, SM;Delwiche, CF;Pace, NR

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从黄石国家公园温泉的未培养生物中获得的核蛋白体RNA序列的系统发育分析揭示了几个新的古生菌群,其中许多是在该王国的先前特征成员之前从crenarchaeal谱系中分离出来的,用这些序列构建的通用系统发育树表明古生菌是单系的,有适度的引导支持。这些数据还显示了低温海洋古菌和一些温泉古菌之间的特殊关系,其中两个环境序列是神秘的:根据数据集和使用的分析方法,这些序列在绿古菌群中有很深的分支,低于绿古菌和Euryarchaeota之间的分支,甚至是真核生物的姐妹群。如果更多的数据证实了后两种位置中的任何一种,那么这些核糖体RNA序列所代表的生物将值得被认可为一个新的王国,暂时命名为“Korarchaeota”。
Phylogenetic analysis of ribosomal RNA sequences obtained from uncultivated organisms of a hot spring in Yellowstone National Park reveals several novel groups of Archaea, many of which diverged from the crenarchaeal line of descent prior to previously characterized members of that kingdom, Universal phylogenetic trees constructed with the addition of these sequences indicate monophyly of Archaea, with modest bootstrap support, The data also show a specific relationship beta een low-temperature marine Archaea and some hot spring Archaea, Two of the environmental sequences are enigmatic: depending upon the data set and analytical method used, these sequences branch deeply within the Crenarchaeota, below the bifurcation between Crenarchaeota and Euryarchaeota, or even as the sister group to Eukaryotes. If additional data confirm either of the latter two placements, then the organisms represented by these ribosomal RNA sequences would merit recognition as a new kingdom, provisionally named ''Korarchaeota.''