Black-Phosphorus-Nanosheet-Reinforced Coating of Implants for Sequential Biofilm Ablation and Bone Fracture Healing Acceleration
Black-Phosphorus-Nanosheet-Reinforced Coating of Implants for Sequential Biofilm Ablation and Bone Fracture Healing Acceleration
复制标题
用于顺序生物膜消融和骨折愈合加速的植入物黑磷纳米片增强涂层
DOI：
10.1021/acsami.2c13566
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发表时间：
2022
影响因子：
9.5
通讯作者：
Xiang Guo
中科院分区：
文献类型：
--
作者：
Bo Yuan;Xin Zhou;Yingke Li;Yin Zhao;Mintao Xue;Qunfeng Guo;Gang Zheng;Xiongsheng Chen;Han Lin;Xiang Guo
无法治愈的植入物相关感染可能会导致灾难性的后果，由于生物膜的存在，抵抗宿主免疫细胞和抗生素的渗透。受纳米医学启发的创新方法，在植入物表面设计创新的多功能仿生涂层系统正变得越来越有吸引力。本文将二维黑磷纳米片（BPs）负载到羟基磷灰石（HA）涂覆的金属植入物上以构建BPs@HA复合涂层。体外和体内实验均证实，BPs@HA涂层具有光热转化效应和原位生物矿化作用，具有良好的消融细菌生物膜和促进骨折愈合的作用。此外，通过绝对定量转录组测序和基因差异表达筛选，确定骨形成和BPs@HA涂层调控的骨髓间充质干细胞（BMSCs）的差异表达基因。功能富集分析表明，BPs通过代谢相关途径有效调节与BMSC分化和骨形成相关的核心标志物的表达。这项工作不仅说明了BPs@HA复合涂层在临床应用中消除细菌和加速骨折愈合的巨大潜力，而且有助于基于绝对定量转录组测序分析来理解成骨生理功能调节的潜在分子机制。
Incurable implant-related infection may cause catastrophic consequences due to the existence of a biofilm that resists the infiltration of host immune cells and antibiotics. Innovative approaches inspired by nanomedicine, e.g., engineering innovative multifunctional bionic coating systems on the surface of implants, are becoming increasingly attractive. Herein, 2D black phosphorus nanosheets (BPs) were loaded onto a hydroxyapatite (HA)-coated metal implant to construct a BPs@HA composite coating. With its photothermal conversion effect andin situbiomineralization, the BPs@HA coating shows excellent performances in ablating the bacterial biofilm and accelerating fracture healing, which were verified through bothin vitroandin vivostudies. Moreover, differentially expressed genes of bone formation and bone mesenchymal stem cells (BMSCs) regulated by the BPs@HA coating were identified using absolute quantitative transcriptome sequencing followed by the screening of gene differential expressions. A functional enrichment analysis reveals that the expression of core markers related to BMSC differentiation and bone formation could be effectively regulated by BPs through a metabolism-related pathway. This work not only illustrates the great potential in clinical application of the BPs@HA composite coating to eliminate bacteria and accelerate bone fracture healing but also contributes to an understanding of the underlying molecular mechanism of osteogenesis physiological function regulation based on an analysis of absolute quantitative transcriptome sequencing.