Microscopic features and untargeted metabolomics reveal special features of the black-patterning in spalted heartwood of Diospyros spp.
Microscopic features and untargeted metabolomics reveal special features of the black-patterning in spalted heartwood of Diospyros spp.
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DOI：
10.1080/02773813.2023.2229300
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发表时间：
2023-07
影响因子：
2
通讯作者：
Zhi Li;Rui Yang;Xiaorui Yang;Huiwen Jia;Jian Qiu
中科院分区：
文献类型：
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作者：
Zhi Li;Rui Yang;Xiaorui Yang;Huiwen Jia;Jian Qiu
摘要石纹心材因其鲜艳的颜色、图案和不腐烂的外观而受到重视。使用显微镜，扫描电子显微镜与能量色散光谱，透射电子显微镜，和非靶向代谢组学，我们的目的是研究一些独特的功能黑色图案。结果表明，球菌可能与散裂心材的产生有关。由于球菌没有攻击细胞壁，所以在剥落的心材中没有腐烂。4-吡哆醇，通常与生物体的细菌刺激有关，被鉴定为与非着色区域相比在黑色图案木材中上调的代谢物。我们确定了四个主要的代谢途径与细菌的活动与黑色图案：戊糖和葡萄糖醛酸相互转换，ATP结合盒转运，组氨酸代谢和植物激素信号转导。这些结果表明，微生物可能在柿心材独特的黑色图案的发展中发挥了重要作用。
Abstract Spalted heartwood is valued for its vibrant colors, patterns, and decay-free appearance. Using microscopy, scanning electron microscopy with energy-dispersive spectroscopy, transmission electron microscopy, and non-targeted metabolomics, we aimed to study some distinctive features of black patterning. The results showed that cocci might be associated with the production of spalted heartwood. There was an absence of decay in the spalted heartwood because the cocci had not aggressively attacked the cell walls. 4-pyridoxine, often associated with the bacterial stimulation of organisms, was identified as an upregulated metabolite in the black-patterned wood compared to non-colored areas. We identified four primary metabolic pathways related to bacterial activity associated with the black patterning: pentose and glucuronate interconversions, ATP-binding cassette transporters, histidine metabolism, and plant hormone signal transduction. These results suggest that microbes might play a significant role in the development of the heartwood’s distinctive black pattern in Diospyros spp.