A high-fat diet reverses improvement in glucose tolerance induced by duodenal-jejunal bypass in type 2 diabetic rats
A high-fat diet reverses improvement in glucose tolerance induced by duodenal-jejunal bypass in type 2 diabetic rats
复制标题
高脂肪饮食可逆转 2 型糖尿病大鼠十二指肠空肠旁路术引起的糖耐量改善
DOI：
10.3760/cma.j.issn.0366-6999.2012.05.032
复制
发表时间：
2012-03-05
影响因子：
6.1
通讯作者：
Hu San-yuan
中科院分区：
文献类型：
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作者：
Liu Shao-zhuang;Sun Dong;Hu San-yuan
背景：减肥手术是成功解决2型糖尿病（T2DM）的方法。然而，在一些减肥手术后最初缓解的患者中观察到T2DM的复发。本研究旨在诱导T2DM大鼠十二指肠-空肠旁路（DJB）后糖尿病改善的逆转，并确定体重变化和肠道激素可能参与的影响。方法采用DJB手术治疗2型糖尿病大鼠模型（每组20只）：非肥胖的Goto-Kakizaki大鼠（GK）和高脂饮食（HFD）和低剂量链脲佐菌素联合诱导的中度肥胖T2DM大鼠（HS大鼠）。对照组为假手术和未治疗的大鼠。然后将所有大鼠随机分为高脂和低脂饮食（LFD）组。测定糖耐量、胰岛素耐量、葡萄糖刺激胰岛素、胰高血糖素样肽-1 （GLP-1）和胰高血糖素样肽-1 （PYY）分泌、摄食量和体重，并与对照组进行比较。结果DJB手术可显著改善GK和HS大鼠的糖耐量。与lfd喂养的大鼠相比，HFD喂养的GK和HS大鼠的糖耐量改善受损，胰岛素耐量再次受损，胰岛素分泌增强失败。djb操作大鼠与对照组大鼠、HFD喂养大鼠与led喂养大鼠的进食量和体重均无显著差异。DJB术后葡萄糖刺激GLP-1和PYY水平显著升高；然而，HFD和lfd喂养的大鼠之间没有显著差异。结论HFD逆转了DJB手术诱导的T2DM大鼠糖耐量的改善，主要是由于胰岛素敏感性的再损伤，但不改变体重、GLP-1和PYY水平。中华医学杂志2012；125 (5): 912 - 919
Background Bariatric surgery offers successful resolution of type 2 diabetes mellitus (T2DM). However, recurrence of T2DM has been observed in a number of patients with initial resolution after bariatric surgery. This study aimed to induce reversal of the improvement of diabetes in T2DM rats after duodenal-jejunal bypass (DJB), and identify the effects of weight changes and gut hormones that might be involved.Methods DJB surgery was performed in two T2DM rat models (n=20 for each group): non-obese Goto-Kakizaki (GK) rats, and moderately-obese T2DM rats induced by a combination of a high-fat diet (HFD) and low-dose streptozotocin (HS rats). The controls were sham-operated and non-treated rats. All rats were then randomly divided into HFD- and low-fat diet (LFD)-fed groups. Glucose tolerance, insulin tolerance, glucose-stimulated insulin, glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) secretion, food intake and body weight were measured and compared with controls.Results DJB surgery resulted in a significant improvement in glucose tolerance in both GK and HS rats fed with either HFD or LFD. In contrast to LFD-fed rats, improved glucose tolerance was impaired in GK and HS rats fed with an HFD, accompanied by re-impairment of insulin tolerance and failure in enhancement of insulin secretion. There was no significant difference in food intake and body weight between DJB-operated and control rats, and between HFD- and LED-fed rats. Glucose-stimulated GLP-1 and PYY levels were significantly increased after DJB surgery; however, they were not significantly different between HFD- and LFD-fed rats.Conclusion An HFD reverses the improvement in glucose tolerance induced by DJB surgery in T2DM rats, primarily ascribing to the re-impairment of insulin sensitivity, but does not change body weight, GLP-1 and PYY levels. Chin Med J 2012;125(5):912-919