A trans-acting RNA as a control switch in Escherichia coli:: DsrA modulates function by forming alternative structures
A trans-acting RNA as a control switch in Escherichia coli:: DsrA modulates function by forming alternative structures
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DOI：
10.1073/pnas.170281497
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发表时间：
2000-08-29
影响因子：
11.1
通讯作者：
Belfort, M
中科院分区：
文献类型：
--
作者：
Lease, RA;Belfort, M
DSRA是大肠杆菌的一个87个核苷酸的调控RNA，通过与两个不同的mRNAs的RNA-RNA相互作用而发挥反式作用，Has和rpos，DSRA对这些转录调控因子具有相反的作用。H-NS水平下降，而rpos(S)水平上升。在这里，我们表明，DSRA增加了HNS mRNA的周转，但稳定了rpos mRNA，无论是直接还是通过对翻译的影响。计算和RNA足迹方法导致了DSRA的精细化结构，以及DSRA与HNS mRNA起始和终止密码子区域相互作用形成同轴堆叠的模型。真核生物中存在类似的两部分相互作用，尽管具有不同的调控后果。相反，DSRA碱基对与rpos RNA翻译操作符是离散的，因此，DSRA的不同结构配置导致了对靶RNA的相反的调控后果。
DsrA is an 87-nucleotide regulatory RNA of Escherichia coli that acts in trans by RNA-RNA interactions with two different mRNAs, has and rpoS, DsrA has opposite effects on these transcriptional regulators. H-NS levels decrease, whereas RpoS (sigma(s)) levels increase. Here we show that DsrA enhances hns mRNA turnover yet stabilizes rpoS mRNA, either directly or via effects on translation. Computational and RNA footprinting approaches led to a refined structure for DsrA, and a model in which DsrA interacts with the hns mRNA start and stop codon regions to form a coaxial stack. Analogous bipartite interactions exist in eukaryotes, albeit with different regulatory consequences. In contrast, DsrA base pairs in discrete fashion with the rpoS RNA translational operator, Thus, different structural configurations for DsrA lead to opposite regulatory consequences for target RNAs.