A transforming growth factor beta 2 (TGF-beta 2)-like immunosuppressive factor in amniotic fluid and localization of TGF-beta 2 mRNA in the pregnant uterus.
A transforming growth factor beta 2 (TGF-beta 2)-like immunosuppressive factor in amniotic fluid and localization of TGF-beta 2 mRNA in the pregnant uterus.
复制标题
羊水中的转化生长因子 β 2 (TGF-β 2) 样免疫抑制因子以及妊娠子宫中 TGF-β 2 mRNA 的定位。
DOI：
10.1084/jem.172.5.1391
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Tomasi,TB
中科院分区：
文献类型：
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作者：
Altman,DJ;Schneider,SL;Thompson,DA;Cheng,HL;Tomasi,TB
本报告描述了一种鼠羊水（MAF）免疫抑制因子，具有类似于转化生长因子0（TGF-R）的特性。MAP因子在刺激AKR-2B细胞的软琼脂集落形成和抑制Mv 1 Lu细胞的胸苷摄取方面表现出TGF-0样活性。我们证明MAP因子的免疫抑制和TGF-β 1样活性都被抗TGF-β 2特异性抗体完全中和，而不被抗TGF-β 1特异性抗血清中和。MAP中的免疫抑制因子是新的，因为它似乎与TGF-02相同或非常密切相关，并且在其天然状态下具有活性。这种活性和抗TGF-β 2-中和因子在中性pH值下在Sephadex上在-70 kD处层析，并且似乎能够与天然羊水中的胎蛋白复合。天然MAP在酸性条件下的层析证明了在BioGel上层析在与成熟的25-kD TGF-0相同的位置的较低分子量蛋白。这种蛋白质具有TGF-0的生物学特性，具有免疫抑制作用。这两种活性都可以用抗TGF-/32中和，但不能用抗TGF-/31或其他抗血清中和。通过北方分析，我们发现妊娠子宫中高水平的TGF-β 2 mRNA（具有很少或没有TGF-β 1）在妊娠第15天左右达到峰值，然后随着分娩的临近在第19天迅速下降。TGF-X32样因子可能在母体免疫、胎儿同种异体移植物的保留以及调节胎儿和新生儿的免疫能力中发挥作用，有几个因素与母体对半同种异体胎儿的耐受性和妊娠期以及胎儿和新生儿的免疫缺陷有关（参考文献1和2）。这包括证据表明，在胎儿和母体组织中的MHC I类和II类抗原表达的缺陷可能在母体耐受性中起作用。不同的T细胞亚群，特别是抑制细胞也与妊娠相关的免疫抑制和免疫营养促进胎儿发育有关（1，2）。新生儿天然抑制细胞抑制免疫应答，并可能参与胎儿和新生儿期的耐受诱导（3）。此外，已经描述了影响胎儿和新生儿免疫发育以及妊娠期间免疫的可溶性抑制因子（1），这些抑制因子可能介导和/或补充细胞抑制因子。我们以前曾报道过（在参考文献4和5中进行了综述）小鼠羊水的免疫抑制作用
This report describes a murine amniotic fluid (MAF) immunosuppressive factor that has properties similar to transforming growth factor 0 (TGF-R). The MAP factor exhibits TGF-0-like activity in stimulating soft agar colony formation by AKR-2B cells and inhibiting thymidine uptake by Mv1Lu cells. We demonstrate that both the immunosuppressive and TGF-#-like activities ofthe MAP factor are completely neutralized by antiTGF-02-specific antibodies and not by anti-TGF-/31-specific antisera. The immunosuppressive factor in MAP is novel in that it appears to be identical or very closely related to TGF-02 and is active in its native state. This active and antiTGF-, 32-neutralizable factor chromatography at-70 kD on Sephadex at neutral pH and appears to be able to complex with ci-fetoprotein in native amniotic fluid. Chromatography of native MAP under acidic conditions demonstrates a lower molecular mass protein that chromatographs on BioGel in the same position as the mature 25-kD TGF-0. This protein has the biological properties of TGF-O and is immunosuppressive. Both of these activities are neutralizable with antiTGF-/32 but not with antiTGF-/31 or other antisera. By Northern analysis, we find high levels of TGF-f2 mRNA (with little or no TGF-fl) in the pregnant uterus that peak around day 15 of gestation and then fall rapidly by day 19 as birth approaches. The TGF-X32-like factor could possibly play a role in maternal immunity, in the retention of the fetal allograft, as well as in regulating fetal and neonatal immunological competence.Several factors have been implicated in the maternal tolerance to the semiallogeneic fetus and the immunological deficiencies described in pregnancy, as well as the fetus and neonate (reviewed in references 1 and 2). This includes evidence that deficiencies in MHC class I and class II antigen expression on fetal and maternal tissues may play a role in maternal tolerance. Different T cell subsets and especially suppressor cells have also been implicated in pregnancy-related immune suppression and in the immunotrophic promotion offetal development (1, 2). Neonatal natural suppressor cells inhibit immune responses and may be involved in tolerance induction in the fetal and neonatal periods (3). In addition, soluble suppressive factors influencing immune development in the fetus and newborn, and immunity during pregnancy, have been described (1), and these may mediate and/or complement the cellular suppressors. We have previously reported (reviewed in references 4 and 5) the immunosuppressive effects of murine amniotic fluid