Physiological and quality responses of Chinese chive leaves to low oxygen atmosphere
Physiological and quality responses of Chinese chive leaves to low oxygen atmosphere
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DOI：
10.1016/j.postharvbio.2003.09.004
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发表时间：
2004-03-01
影响因子：
7
通讯作者：
Chachin, K
中科院分区：
文献类型：
--
作者：
Imahori, Y;Suzuki, Y;Chachin, K
将收获的韭菜叶片分别在0、1、3% O(2)（平衡N(2)）和20℃空气中贮存7 d，研究低O(2)环境对韭菜叶片生理和品质的影响。叶片在空气中第5天出现黄变，而低氧(2)处理延缓了黄变，延缓了伴随叶片衰老的叶绿素和蛋白质降解。低0℃贮藏期间，叶片的呼吸速率明显低于对照。然而，在0% O(2)时，不良的异味被诱导，并且在储存的叶片中出现明显的厌氧损伤，可能是由于组织中乙醛和乙醇的大量积累。在1% O(2)、3% O(2)或空气中储存期间，乙醛和乙醇的含量非常低。在0% O(2)时，乙醇和乙醛在叶组织中迅速积累。丙酮酸脱羧酶（PDC）活性在0或1% O(2)处理下显著升高，而在3% O(2)处理下仅略高于对照。乙醇脱氢酶（ADH）活性在1%或3% O(2)处理下显著提高，而在0% O(2)处理下仅略高于对照。贮藏期间ADH的活性约为PDC的250倍。ADH同工酶的变化与ADH活性的变化密切相关。讨论了利用低氧(2)气氛有助于保持韭菜叶质量的潜力。(C) 2003 Elsevier B.V.版权所有
Harvested leaves of Chinese chive were stored in 0, 1 or 3% O(2) (balance N(2)), or air for 7 days at 20degreesC to determine the effects of low O(2) atmospheres on their physiology and quality. Leaf yellowing was visible at day 5 in air, whereas low O(2) treatment delayed yellowing and retarded chlorophyll and protein degradation that accompanied leaf senescence. The respiration rates of leaves stored at low 0, atmospheres were substantially lower than those of the control during storage. However, at 0% O(2), undesirable off-odors were induced and visible anaerobic injury appeared in stored leaves, presumably due to a high accumulation of acetaldehyde and ethanol in the tissue. The contents of acetaldehyde and ethanol were very low during storage at 1% O(2), 3% O(2), or air. At 0% O(2), ethanol and to a lesser extent acetaldehyde, rapidly accumulated in the leaf tissue. Pyruvate decarboxylase (PDC) activity greatly increased in leaves exposed to 0 or 1% O(2), while its activity in leaves exposed to 3% O(2) was only slightly higher than that of the control. Alcohol dehydrogenase (ADH) activity greatly increased in leaves exposed to 1 or 3% O(2), while its activity in leaves exposed to 0% O(2) was only slightly higher than that of the control. The activity of ADH was about 250 times that of PDC during storage. Changes in ADH isozymes correlated well with changes in ADH activity. The potential for using low O(2) atmospheres to help in maintaining the quality of Chinese chive leaves is discussed. (C) 2003 Elsevier B.V. All rights reserved.