Germ-line transformation of Arabidopsis lasiocarpa
Germ-line transformation of Arabidopsis lasiocarpa
复制标题
毛果拟南芥种系转化
DOI：
10.1023/a:1016633617908
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发表时间：
2001
影响因子：
3
通讯作者：
B. Tague
中科院分区：
文献类型：
--
作者：
B. Tague
植物体内转化方法已经开辟了转化目前不存在再生方案的植物物种的可能性。在这项研究中，适合的种系转化方法开发的拟南芥进行了检查，在拟南芥科，以前没有被转化的四个类群：拟南芥griffithiana，拟南芥lasiocarpa，拟南芥petraea和荠菜囊，巴斯德。获得了大量的A.毛果草A.转化在表型和分子水平上，对于稳定转化的品系和分离T-DNA插入片段的回交品系，证实了毛果芸香属的突变。影响A.毛果苔属也进行了研究。与A.拟南芥、蔗糖和表面活性剂是高水平转化所必需的，尽管它们的合适浓度对于A.毛果草早期版本的接种培养基中存在的其他组分对转化效率几乎没有影响。真空渗透（而不是简单的浸花）导致更高的转化率，并没有严重影响种子生产在A。毛果草鉴定对种系转化敏感的物种将有助于确定将该技术应用于更多的非转基因物种的重要因素。
In planta transformation methods have opened up the possibility of transforming plant species for which no regeneration protocols currently exist. In this study, the suitability of the germ-line transformation method developed for Arabidopsis thaliana was examined for four taxa in the Brassicaceae that have not been previously transformed: Arabidopsis griffithiana, Arabidopsis lasiocarpa, Arabidopsis petraea and Capsella bursa-pastoris. Numerous transformants were obtained for A. lasiocarpa. Transformation of A. lasiocarpa was confirmed at the phenotypic and molecular levels for stably transformed lines and for backcrossed lines segregating the T-DNA insert. Parameters affecting transformation efficiency of A. lasiocarpa were also explored. As with A. thaliana, sucrose and surfactant in the inoculation medium are required for high levels of transformation, although the suitable concentrations of these are different for A. lasiocarpa. Other components present in earlier versions of the inoculation medium had little effect on transformation efficiency. Vacuum infiltration (rather than simple floral dipping) led to higher rates of transformation and did not seriously affect seed production in A. lasiocarpa. Identification of species susceptible to germ-line transformation will aid in determining the factors important for applying this technology to more recalcitrant species.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs