Nanopore single-molecule detection of circulating microRNAs.
Nanopore single-molecule detection of circulating microRNAs.
复制标题
循环 microRNA 的纳米孔单分子检测。
DOI：
10.1007/978-1-62703-453-1_21
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Wang, Yong
中科院分区：
文献类型：
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作者：
Gu, Li-Qun;Wang, Yong
microRNAs（miRNAs）是一类在细胞活动的各个方面发挥重要作用的微小非编码RNA。miRNA的功能性表达破坏了正常的生物学过程，导致包括癌症在内的各种疾病的发展。循环中的miRNAs作为一种生物标志物，具有非侵入性疾病检测的潜力。这就需要开发新的技术，以短的检测时间和可负担的成本准确地检测miRNA。我们提出了一种纳米孔单分子方法，用于准确、无标记地检测循环miRNA，而不扩增靶miRNA。这个简明的方案描述了如何在RNA提取中设计蛋白质纳米孔来定量靶miRNA，并在最后讨论了纳米孔方法用于miRNA检测的优势，挑战和广泛影响。
MicroRNAs (miRNAs) are a class of tiny noncoding RNAs that play an important role in regulating every aspect of cellular activities. Dysfunctional expression of miRNAs disrupts normal biological processes, leading to the development of various diseases including cancer. Circulating miRNAs are being investigated as biomarkers with a potential for noninvasive disease detection. This demands the development of new technologies to accurately detect miRNAs with short assay time and affordable cost. We have proposed a nanopore single-molecule method for accurate, label-free detection of circulating miRNAs without amplification of the target miRNA. This concise protocol describes how to device a protein nanopore to quantify target miRNAs in RNA extraction, and discusses at the end the advantages, challenges, and broad impact of the nanopore approach for miRNA detection.