Follow-up of avidity and titre of anti-myeloperoxidase antibodies in sera from patients with propylthiouracil-induced vasculitis
Follow-up of avidity and titre of anti-myeloperoxidase antibodies in sera from patients with propylthiouracil-induced vasculitis
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DOI：
10.1111/j.1365-2265.2007.02770.x
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发表时间：
2007-04-01
影响因子：
3.2
通讯作者：
Wang, Hai-yan
中科院分区：
文献类型：
--
作者：
Gao, Ying;Chen, Min;Wang, Hai-yan
目的 已知丙硫氧嘧啶 (PTU) 可诱导髓过氧化物酶抗中性粒细胞胞浆抗体 (MPO-ANCA) 阳性血管炎。我们前期的研究表明，MPO-ANCA亲和力的增加可能与PTU诱导的ANCA患者临床血管炎的发生有关。本研究旨在追踪 PTU 诱导的 ANCA 相关系统性血管炎 (AASV) 患者序贯血清中抗 MPO 抗体的亲合力和滴度。方法 6 名 PTU 诱导的血管炎患者纳入本研究。收集活动期和缓解期的系列血清。通过抗原抑制酶联免疫吸附测定 (ELISA) 评估 MPO-ANCA 亲合力，亲和力常数 (aK) 确定为液相中 MPO 摩尔浓度的倒数，导致固相 ELISA 中抗 MPO 抗体与 MPO 结合的 50% 抑制。 MPO-ELISA 中使用系列血清稀释液测定 MPO-ANCA 滴度。结果 停止 PTU 并开始免疫抑制治疗后，所有患者血清中 MPO-ANCA 的亲和力和滴度在随访期间均显着下降，且亲合力下降的速度远快于滴度。结论我们的研究表明，抗 MPO 抗体的亲和力可能比滴度与临床血管炎更密切相关。
Objective Propylthiouracil (PTU) has been known to induce myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA) positive vasculitis. Our previous study indicated that the increase of avidity of MPO-ANCA might be associated with the occurrence of clinical vasculitis in patients with PTU-induced ANCA. The current study aimed to follow-up the avidity and titre of anti-MPO antibodies in sequential sera from patients with PTU-induced ANCA-associated systemic vasculitis (AASV).Methods Six patients with PTU-induced vasculitis were enrolled in the current study. Serial sera in both active phase and in remission were collected. MPO-ANCA avidity was assessed by antigen-inhibition enzyme-linked immunosorbent assays (ELISAs), and avidity constant (aK) was determined as the reciprocal value of the MPO molar concentration in the liquid phase resulting in 50% inhibition of anti-MPO antibody binding to MPO in solid phase ELISA. Titres of MPO-ANCA were determined by using serial serum dilutions in MPO-ELISA.Results After cessation of PTU and initiation of immunosuppressive therapy, the avidity and titre of MPO-ANCA decreased significantly during follow-up in sera from all the patients, and the avidity decreased much more quickly than the titres.Conclusion Our study indicates that avidity of anti-MPO antibodies might be more closely associated with clinical vasculitis than titre.