CHARACTERIZATION OF THE FLIL GENE IN THE FLAGELLAR REGULON OF ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM
CHARACTERIZATION OF THE FLIL GENE IN THE FLAGELLAR REGULON OF ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.176.8.2308-2311.1994
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发表时间：
1994-04-01
影响因子：
3.2
通讯作者：
MACNAB, RM
中科院分区：
文献类型：
--
作者：
RAHA, M;SOCKETT, H;MACNAB, RM
fliL是一个功能未知的小基因，位于鼠伤寒沙门氏菌和大肠杆菌的大鞭毛操纵子的起始部分。一个自发的S.鼠伤寒沙门氏菌，含有一个移码突变约40%的3'端的基因，是适度的运动，但群集差，这表明FliL可能是一个组件的鞭毛马达或开关。然而，E.大肠杆菌基因，包括基本上完全缺失，对运动性或趋化性几乎没有影响。因此，FliL似乎没有在鞭毛结构或功能中发挥重要作用，因此不太可能是马达或开关的组成部分;基因截短对运动性的影响可能是间接的。
fliL is a small gene of unknown function that lies within the beginning of a large flagellar operon of Salmonella typhimurium and Escherichia coli. A spontaneous fliL mutant of S. typhimurium, containing a frameshift mutation about 40% from the 3' end of the gene, was moderately motile but swarmed poorly, suggesting that FliL might be a component of the flagellar motor or switch. However, in-frame deletions of the E. coli gene, including an essentially total deletion, had little or no effect on motility or chemotaxis. Thus, FliL does not appear to have a major role in flagellar structure or function and is therefore unlikely to be a component of the motor or switch; the effect on motility caused by truncation of the gene is probably an indirect one.