Circadian variation in concentration of anti-Mullerian hormone in regularly menstruating females: relation to age, gonadotrophin and sex steroid levels
Circadian variation in concentration of anti-Mullerian hormone in regularly menstruating females: relation to age, gonadotrophin and sex steroid levels
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DOI：
10.1093/humrep/deq380
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发表时间：
2011-03-01
影响因子：
6.1
通讯作者：
Giwercman, Aleksander
中科院分区：
文献类型：
--
作者：
Bungum, Leif;Jacobsson, Anna-Karin;Giwercman, Aleksander
背景：抗苗勒氏管激素（AMH）是卵巢储备的一个有前途的标志物。本研究的目的是评估 AMH 的昼夜节律变化，并评估其临床相关性和生物学方面作为年龄和参与调节 AMH 分泌的其他内分泌机制的影响。方法：19 名体重指数低于 30 kg/m(2) 的健康、不吸烟、月经规律的女性志愿者，其中 10 名 20-30 岁（A 组）和 9 名 35-45（B组）被包括在内。血液采样，于上午 8:00 开始。米。在月经周期的第 2-6 天，每隔一小时持续一次，直到上午 8:00。米。第二天。测定血清AMH、FSH、LH、孕酮、雌二醇水平。结果：上午8：00。米。以调查第一天的值作为参考，汇总数据中的平均浓度显示凌晨 4: 00 的水平明显较低。米。 (P = 0.021) 和 6: 00 a。米。 (P = 0.005)，最大平均差异为 1.9 pmol/l (10.6%)。在两个年龄组中都看到了相同的模式。包括两个年龄组在内，AMH 水平的整体昼夜变化未达到统计学显着性（P = 0.059）。 24 小时内 AMH 和 LH 浓度之间存在显着正相关 (P < 0.001)。结论：深夜血清 AMH 水平略有下降似乎不具有临床相关性。 LH 和 AMH 水平的共同变化可能表明后者激素和促性腺激素的联合调节机制。
BACKGROUND: Anti-Mullerian hormone (AMH) is a promising marker of ovarian reserve. The aim of the study is to assess the circadian variation in AMH, and to evaluate its clinical relevance and biological aspects as an effect of age and other endocrine mechanisms involved in the regulation of AMH secretion.METHODS: Nineteen healthy non-smoking, regularly menstruating female volunteers with body mass index below 30 kg/m(2), 10 aged 20-30 years (Group A) and 9 aged 35-45 (Group B) were included. Blood sampling, initiated at 8: 00 a. m. on Days 2-6 of the menstrual cycle, was continued every second hour until 8: 00 a. m. the following day. Serum levels of AMH, FSH, LH, progesterone and estradiol were measured.RESULTS: With 8: 00 a. m. values at the first day of investigation as a reference, the mean concentrations in the pooled data revealed a significantly lower level at 4: 00 a. m. (P = 0.021) and 6: 00 a. m. (P = 0.005) with a maximum mean difference of 1.9 pmol/l (10.6%). The same pattern was seen in both the age groups. Including both the age groups, the overall circadian variation of the AMH levels did not reach statistical significance (P = 0.059). A significant positive correlation between AMH and LH concentration was seen over the 24-h period (P < 0.001).CONCLUSIONS: A slight decrease in serum AMH levels during the late night appears not clinically relevant. Co-variation in the levels of LH and AMH might indicate joint regulatory mechanisms for the latter hormone and gonadotrophins.