Foliar-applied glutathione activates zinc transport from roots to shoots in oilseed rape
Foliar-applied glutathione activates zinc transport from roots to shoots in oilseed rape
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DOI：
10.1016/j.plantsci.2018.10.018
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发表时间：
2019-06-01
期刊：
影响因子：
5.2
通讯作者：
Ohkama-Ohtsu, Naoko
中科院分区：
文献类型：
--
作者：
Nakamura, Shin-ichi;Wongkaew, Arunee;Ohkama-Ohtsu, Naoko
谷氨酰胺是一种参与植物代谢各个方面的三肽。我们研究了还原型谷胱甘肽（GSH），适用于特定地点的植物，影响锌（Zn）的分布和行为的油菜植物（甘蓝型油菜）水培。叶面喷施GSH显著提高了地上部Zn含量和Zn向地上部的转运率，并显著提高了根细胞胞液中Zn含量和木质部Zn负荷。值得注意的是，微阵列分析显示，基因编码果胶甲酯酶在根叶GSH治疗后上调。我们的结论是，某些生理信号触发响应叶面施加GSH通过筛管和根细胞中的功能，这反过来又增加了锌的可用性，从他们的细胞壁中释放锌。因此，根到地上部的锌转运被激活，地上部锌积累量显着增加。
Glutathione is a tripeptide involved in diverse aspects of plant metabolism. We investigated how the reduced form of glutathione, GSH, applied site-specifically to plants, affects zinc (Zn) distribution and behavior in oilseed rape plants (Brassica napus) cultured hydroponically. Foliar-applied GSH significantly increased the Zn content in shoots and the root-to-shoot Zn translocation ratio; furthermore, this treatment raised the Zn concentration in the cytosol of root cells and substantially enhanced Zn xylem loading. Notably, microarray analysis revealed that the gene encoding pectin methylesterase was upregulated in roots following foliar GSH treatment. We conclude that certain physiological signals triggered in response to foliar-applied GSH were transported via sieve tubes and functioned in root cells, which, in turn, increased Zn availability in roots by releasing Zn from their cell wall. Consequently, root-to-shoot translocation of Zn was activated and Zn accumulation in the shoot was markedly increased.