CROSS SPECIFICITY IN SOME VERTEBRATE AND INSECT GLUTATHIONE-TRANSFERASES WITH METHYL PARATHION (DIMETHYL P-NITROPHENYL PHOSPHOROTHIONATE), 1-CHLORO-2,4-DINITROBENZENE AND S-CROTONYL-N-ACETYLCYSTEAMINE AS SUBSTRATES
CROSS SPECIFICITY IN SOME VERTEBRATE AND INSECT GLUTATHIONE-TRANSFERASES WITH METHYL PARATHION (DIMETHYL P-NITROPHENYL PHOSPHOROTHIONATE), 1-CHLORO-2,4-DINITROBENZENE AND S-CROTONYL-N-ACETYLCYSTEAMINE AS SUBSTRATES
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DOI：
10.1042/bj1350385
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发表时间：
1973-01-01
影响因子：
4.1
通讯作者：
SPEIR, TW
中科院分区：
文献类型：
--
作者：
CLARK, AG;SMITH, JN;SPEIR, TW
1.用（NH_4）_2SO_4沉淀法从羊、大鼠、小鼠肝匀浆和蟑螂、家蝇、幼虫肝匀浆中分离出催化GSH与甲基对硫磷、1-氯-2，4-二硝基苯和S-巴豆酰-N-乙酰半胱胺反应的酶。2.这些物种的制备物的电聚焦分离了许多区域，每个区域催化GSH与所有三种底物的反应。3. CM-纤维素上的离子交换色谱法也分离出了许多组分，其中对三种底物的活性一致。4.在这两种分离方法的活动模式是一致的存在下，在所有物种的几个GSH转移酶，每个都有一定程度的交叉特异性对三个基板。
1. Enzymes catalysing the reaction between GSH and methylparathion (dimethylp-nitrophenyl phosphorothionate), 1-chloro-2,4-dinitrobenzene andS-crotonyl-N-acetylcysteamine were separated by (NH4)2SO4precipitation from homogenates of sheep, rat and mouse livers and from homogenates of cockroaches, houseflies and grass grubs. 2. Electrofocusing of the preparations from each of these species separated a number of zones, each of which catalysed the reaction of GSH with all three substrates. 3. Ion-exchange chromatography on CM-cellulose also separated a number of fractions in which activity towards the three substrates coincided. 4. In both separation methods patterns of the activities were consistent with the presence in all species of several GSH transferases each having a degree of cross specificity towards the three substrates.