Hyperphagia induced by sucrose: Relation to circulating and CSF glucose and corticosterone and orexigenic peptides in the arcuate nucleus
Hyperphagia induced by sucrose: Relation to circulating and CSF glucose and corticosterone and orexigenic peptides in the arcuate nucleus
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DOI：
10.1016/j.pbb.2010.10.008
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Leibowitz, S. F.
中科院分区：
文献类型：
--
作者：
Gaysinskaya, V. A.;Karatayev, O.;Leibowitz, S. F.
众所周知，与富含淀粉的饮食相比，富含蔗糖的饮食会刺激慢性疾病患者的暴饮暴食。本研究在正常体重大鼠中建立了一个急性“预负荷试验餐”范例，用于证明蔗糖诱导的摄食过多，并研究介导这种行为现象的可能机制。在这种急性模式中，首先给予大鼠小（15 kcal）蔗糖预负荷（30%蔗糖）30分钟，与等量的淀粉预负荷（25%淀粉和5%蔗糖）相比，然后允许自由食用随后的实验室食物测试餐。当与能量密度和适口性相等的淀粉预负荷相比时，蔗糖预负荷在预负荷结束后60至120分钟之间发生的随后的测试餐中持续增加食物摄入。激素，代谢物和下丘脑肽的测量之前，这种摄食过多显示蔗糖与淀粉组之间的显着差异，可能有助于增加食物摄入量。而蔗糖组相比，淀粉组在预负荷后立即（在10分钟），血清和脑脊液（CSF）中的葡萄糖水平升高沿着同时弓状核（ARC）中神经肽Y（NPY）和刺鼠相关蛋白（AgRP）的表达减少，随后的影响（在30-60分钟）之前的测试餐摄食量是相反的。沿着较低的葡萄糖水平，它们包括显著升高的血清和CSF皮质酮水平以及ARC中NPY和AgRP的mRNA水平。除了建立一个动物模型，蔗糖诱导的暴食症，这些结果表明外周和中枢机制，可能介导这种行为现象。(C)2010年爱思唯尔公司All rights reserved.
Sucrose-rich diets compared to starch-rich diets are known to stimulate overeating under chronic conditions. The present study in normal-weight rats established an acute "preload-to-test meal" paradigm for demonstrating sucrose-induced hyperphagia and investigating possible mechanisms that mediate this behavioral phenomenon. In this acute paradigm, the rats were first given a small (15 kcal) sucrose preload (30% sucrose) for 30 min compared to an equicaloric, starch preload (25% starch with 5% sucrose) and then allowed to freely consume a subsequent test meal of lab chow. The sucrose preload, when compared to a starch preload equal in energy density and palatability, consistently increased food intake in the subsequent test meal occurring between 60 and 120 min after the end of the preload. Measurements of hormones, metabolites and hypothalamic peptides immediately preceding this hyperphagia revealed marked differences between the sucrose vs starch groups that could contribute to the increase in food intake. Whereas the sucrose group compared to the starch group immediately after the preload (at 10 min) had elevated levels of glucose in serum and cerebrospinal fluid (CSF) along with reduced expressions of neuropeptide Y (NPY) and agouti-related protein (AgRP) in the arcuate nucleus (ARC), the subsequent effects (at 30-60 min) just preceding the test meal hyperphagia were the reverse. Along with lower levels of glucose, they included markedly elevated serum and CSF levels of corticosterone and mRNA levels of NPY and AgRP in the ARC. In addition to establishing an animal model for sucrose-induced hyperphagia, these results demonstrate peripheral and central mechanisms that may mediate this behavioral phenomenon. (C) 2010 Elsevier Inc. All rights reserved.