GROWTH AND TRANSPARENCY IN THE LENS, AN EPITHELIAL TISSUE, STIMULATED BY PULSES OF PDGF
GROWTH AND TRANSPARENCY IN THE LENS, AN EPITHELIAL TISSUE, STIMULATED BY PULSES OF PDGF
复制标题
DOI：
10.1126/science.3187521
复制
发表时间：
1988-11-04
期刊：
影响因子：
56.9
通讯作者：
CLARK, JI
中科院分区：
文献类型：
--
作者：
BREWITT, B;CLARK, JI
大鼠晶状体在出生后早期发育过程中经历急剧生长。镜片重量在 26 天内增加了 23 倍。每日增长率在 0% 到 87% 之间波动。设计了一种新的培养系统来研究发育过程中的生长振荡。体外晶状体的生长和透明度需要在 HL-1 无血清培养基中脉冲输送血小板衍生生长因子 (PDGF)。连续递送含有PDGF的HL-1培养基或脉冲递送不含PDGF的HL-1培养基导致晶状体混浊并且不生长。这些结果提供了直接证据，表明 PDGF 刺激上皮组织，并且在大鼠晶状体的正常发育过程中发生生长振荡。
The rat lens undergoes dramatic growth during early postnatal development. Lens weight increased by a factor of 23 in 26 days. Growth rate per day oscillated between 0 and 87 percent. A new culture system was designed to study the oscillations in growth during development. Lens growth and transparency in vitro required pulsatile delivery of platelet-derived growth factor (PDGF) in HL-1 serum-free medium. Continuous delivery of HL-1 medium with PDGF or pulsatile delivery of HL-1 medium without PDGF resulted in lens opacity and no growth. These results provide direct evidence that PDGF stimulates an epithelial tissue and that oscillations in growth occur during normal development of the rat lens.