Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering
Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering
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DOI：
10.1252/jcej.40.51
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发表时间：
2007-01-01
影响因子：
0.8
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ino, Kosuke;Ito, Akira;Honda, Hiroyuki
组织工程的主要挑战之一仍然是体外血管化3D移植的构建。我们最近提出了一种新技术，称为“磁力组织工程”（磁力- te），可以在不使用支架的情况下建立三维（3D）组织。磁铁矿阳离子脂质体（mcl）含有10纳米的磁铁矿纳米颗粒，以改善磁铁矿纳米颗粒在靶细胞中的积累，用于磁性标记正常的人真皮成纤维细胞（ndfs）。将磁性标记的ndfs播种到超低附着板上。当在平板下面放置一块磁铁时，细胞聚集在孔的底部。24小时孵育后，细胞形成片状结构，在NHDF片内含有主要的真皮细胞外基质（ECM）成分（纤维连接蛋白和I型胶原）。采用两种方法将人脐静脉内皮细胞（HUVECs）与NHDF片共培养：将HUVECs与NHDF片混合，经Mag-TE使其形成细胞片；或NHDF片由Mag-TE和huvec随后播种到NHDF片。这些方法在短期3D共培养后，在NHDF片内或表面形成了类似早期毛细血管的管状haec，从而表明magg -te可能有助于构建涉及毛细血管的3D组织。
One of the major challenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NHDF sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NHDF sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.