The seating effect of fibrin glue against alveolar air leakage evaluated up to 48 h; comparison between different methods of application
The seating effect of fibrin glue against alveolar air leakage evaluated up to 48 h; comparison between different methods of application
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DOI：
10.1016/j.ejcts.2005.02.044
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Kawamura, M;Gika, M;Kobayashi, K
目的：几乎没有实验证据表明纤维蛋白密封剂实际上可以承受多大的气道正压，特别是这种效果如何随时间变化。在本研究中，我们通过实验评估了应用后48 h内纤维蛋白胶对肺泡漏气的密封效果。方法：使用比格犬（n=48）。在开胸手术下，在肺表面上制作约5 X 10 mm缺损（2 mm深）。用三种方法将粘合胶密封剂应用于该缺陷。在摩擦和喷雾方法中，纤维蛋白原被摩擦，随后纤维蛋白原和凝血酶溶液两者被喷雾。双层法先滴入纤维蛋白原，再滴入凝血酶。还测试了涂覆有纤维蛋白原和凝血酶（TachoComb）的胶原纤维。在应用后0、3、6、12、24和48 h（每个时间点n=6），测量每个固定缺陷产生漏气的最小气道正压（固定破裂压力，cmH（2）O）。结果：在应用方法中，密封破坏压力随时间增加。在6 h时，两种方法之间的差异不显著，但RS中的三个缺陷达到70 cmH（2）0，最大测试压力，而其他两种方法中没有。在12 h时，RS的密封破坏压力显著高于其他两种方法（摩擦和喷雾法vs TachoComb; 66 +/- 3 vs 47 +/- 17，P=0.047，摩擦和喷雾法vs双层法; 66 3 vs 42 +/- 18，P=0.024）。24 h后，所有方法的阀座压力均接近70 cmH（2）O。结论：结果表明，纤维蛋白胶的密封效果在其应用后长达12 h相对不稳定。摩擦法和喷涂法比其他两种方法能更快地使纤维蛋白座达到其完全强度。(c)2005 Elsevier B. V.保留所有权利。
Objective: There is little experimental evidence to show how much positive airway pressure fibrin sealants can actually withstand, and in particular, how this effect changes over time. In the present study, we experimentally evaluated the seating effect of fibrin glue against alveolar air leakage up to 48 h after application. Methods: Beagles were used (n=48). Under thoracotomy, approximately 5 X 10 mm defects (2 mm depth) were made on the lung surface. Fibrin glue sealants were applied to this defect in three ways. In rubbing and spray method, fibrinogen was rubbed, followed by spraying of both fibrinogen and thrombin solutions. In double layer method, fibrinogen was dripped, followed by thrombin. Collagen fleece, coated with fibrinogen and thrombin (TachoComb) was also tested. The minimum positive airway pressure which produced air leakage was measured for each seated defect (seat breaking pressure, cmH(2)O) at 0, 3, 6, 12, 24, and 48 h after application (n=6 at each time point). Results: The seal-breaking pressure increased over time in at[ of the application methods. At 6 h, differences between methods were not significant but three defects in RS reached 70 cmH(2)0, the maximum pressure tested, compared with none in other two methods. At 12 h, the seal-breaking pressure was significantly higher in RS compared with the other two methods (rubbing and spray method vs TachoComb; 66 +/- 3 vs 47 +/- 17, P=0.047, rubbing and spray method vs double layer method; 66 3 vs 42 +/- 18, P=0.024). Beyond 24 h, seating pressure reached close to 70 cmH(2)O in ail the methods. Conclusions: The results show that the seating effect of fibrin glue is relatively unstable up to 12 h after its application. Rubbing and spray method may help the fibrin seat to reach its full strength faster compared with the other two methods. (c) 2005 Elsevier B.V. All rights reserved.