Microbial and metabolomic profiles in correlation with depression and anxiety co-morbidities in diarrhoea-predominant IBS patients
Microbial and metabolomic profiles in correlation with depression and anxiety co-morbidities in diarrhoea-predominant IBS patients
复制标题
腹泻为主的 IBS 患者的微生物和代谢组学特征与抑郁和焦虑共病的相关性
DOI：
10.1186/s12866-020-01841-4
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发表时间：
2020-06-17
期刊：
影响因子：
4.2
通讯作者：
Zuo, Xiu-li
中科院分区：
文献类型：
--
作者：
Liu, Tong;Gu, Xiang;Zuo, Xiu-li
背景肠易激综合征（IBS）的心理共病已被广泛认可，而鲜为人知的是肠道微生物和宿主代谢变化的作用，在临床和心理症状在IBS.ResultsA共70肠易激综合征（IBS-D）患者和46名健康对照者参加了这项研究。结果显示，IBS-D组粪菌群以粪杆菌属（Faecalibacterium）减少（P =0.034）、直肠真杆菌属（Eubacterium rectale）减少（P =0.048）、亚长粒菌（Subdoligranulum）减少（P = 0.041）、普雷沃菌（Prevotella）增加（P =0.041）为主。在IBS-D患者中，O-脲基-L-丝氨酸、3，4-二羟基苯磺酸和（R）-2-羟基戊二酸的尿浓度较低。我们进一步建立肠道微生物丰度、分化代谢物数量与临床参数之间的相关矩阵，辨证分型与IBS严重程度呈负相关（r=-0.285，P = 0.017），焦虑Roseburia与IBS严重程度呈负相关（r =-0.298，P = 0.012）。20种代谢物与焦虑或抑郁水平相关，包括3，4-二羟基扁桃醛与SAS（r=-0.383，P = 0.001）、1-甲基黄嘌呤与SDS（r=-0.347，P = 0.004）和1D-手性肌醇与SAS（r=-0.336，P = 0.005）。在微生物代谢物的关系分析，3，4-二羟基扁桃醛和1-甲基黄嘌呤呈负相关的相对丰度ofClostridiumsensu stricto.ConclusionsOur研究结果表明，改变微生物和代谢物的配置文件与临床和精神症状的IBS-D患者，这可能会提供进一步调查的见解。
BackgroundPsychological co-morbidities in irritable bowel syndrome (IBS) have been widely recognized, whereas less is known regarding the role of gut microbial and host metabolic changes in clinical and psychological symptoms in IBS.ResultsA total of 70 diarrhoea-predominant IBS (IBS-D) patients and 46 healthy controls were enrolled in this study. Stool and urine samples were collected from both groups for 16S rRNA gene sequencing and metabolomic analysis.The results showed that fecal microbiota in IBS-D featured depletedFaecalibacterium(adjustedP =0.034),Eubacterium rectalegroup (adjustedP =0.048),Subdoligranulum(adjustedP =0.041) and increasedPrevotella(adjustedP =0.041). O-ureido-L-serine, 3,4-dihydroxybenzenesulfonic acid and (R)-2-Hydroxyglutarate demonstrated lower urinary concentrations in IBS-D patients. We further built correlation matrices between gut microbe abundance, differentiated metabolite quantities and clinical parameters.Dialistermanifested negative association with IBS severity (r= − 0.285,P= 0.017), anxiety (r= − 0.347,P= 0.003) and depression level (r= − 0.308,P= 0.010).Roseburiawas negatively associated with IBS severity (r= − 0.298,P= 0.012). Twenty metabolites correlated with anxiety or depression levels, including 3,4-dihydroxymandelaldehyde with SAS (r= − 0.383,P= 0.001), 1-methylxanthine with SDS (r= − 0.347,P= 0.004) and 1D-chiro-inositol with SAS (r= − 0.336,P= 0.005). In analysis of microbe-metabolite relationship, 3,4-dihydroxymandelaldehyde and 1-methylxanthine were negatively correlated with relative abundance ofClostridiumsensu stricto.ConclusionsOur findings demonstrated altered microbial and metabolomic profiles associated with clinically and psychological symptoms in IBS-D patients, which may provide insights for further investigations.