A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
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DOI：
10.1371/journal.pone.0027624
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发表时间：
2011-11-16
期刊：
影响因子：
3.7
通讯作者：
Rezsohazy, Rene
中科院分区：
文献类型：
--
作者：
Diman, Nata Y. S. -G.;Remacle, Sophie;Rezsohazy, Rene
先前产生的转基因小鼠系携带的β-半乳糖苷酶报告基因的近端2 kb的Hoxa 3启动子控制的调查的监管线索，在小鼠中的Hoxa 3表达。从胚胎日（E）8.0至E15.5转基因胚胎的检查显示，在发育中的心脏区域限制的报告活性。事实上，转基因表达特异性地描绘了来自三个不同谱系的细胞：第二心脏领域的亚群，有助于流出道心肌，心脏神经嵴细胞和咽内胚层。视黄酸（RA）信号传导途径的操作表明，RA是转基因正确表达所必需的。因此，该转基因系可作为特别感兴趣的心脏传感器系，用于流出道发育的进一步分析。
A transgenic mouse line harbouring a beta-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.