Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats
Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats
复制标题
冷冻疗法减少大鼠缺血/再灌注后骨骼肌损伤
作者：
G. Puntel;N. Carvalho;Fernando Dobrachinski;A. Salgueiro;R. Puntel;V. Folmer;N. V. Barbosa;L. Royes;J. Rocha;F. Soares
本研究旨在分析冷冻治疗对大鼠缺血再灌注(I/R)腓肠肌生化和形态的影响。雄性Wistar大鼠40只，随机分为对照组和I/R组，按是否接受冷冻治疗分为两组。再灌注期后，进行生化和形态分析。冷冻治疗后，在I/R肌肉中观察到硫代巴比妥酸反应物质和二氯荧光素氧化水平的减少。冷冻治疗I/R肌肉的效果也最小，如细胞存活率降低，非蛋白硫醇水平和钙ATPase活性降低，过氧化氢酶活性增加。冷冻治疗还限制了线粒体功能障碍，减少了I/R肌肉中中性粒细胞的存在，这一效果得到了冷冻治疗后I/R肌肉髓过氧化物酶活性降低的证实。冷冻治疗的效果与炎症反应强度的降低以及线粒体功能障碍的减少有关。
The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused (I/R) gastrocnemius muscle of rats. Forty male Wistar rats were divided into control and I/R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid‐reactive substances and dichlorofluorescein oxidation levels were observed in I/R muscle. Cryotherapy in I/R muscle also minimized effects such as decreased cellular viability, levels of non‐protein thiols and calcium ATPase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I/R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I/R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction.
影响因子：
--
作者：
GRISHAM, MB;HERNANDEZ, LA;GRANGER, DN
通讯作者：
GRANGER, DN
影响因子：
7.4
作者：
Zaidi, A;Michaelis, ML
通讯作者：
Michaelis, ML