A Novel Dual Channel Fluorescent Probe for Ca2+ and Zn2+ Based on a Coumarin Schiff Base
A Novel Dual Channel Fluorescent Probe for Ca2+ and Zn2+ Based on a Coumarin Schiff Base
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一种基于香豆素席夫碱的新型 Ca2 和 Zn2 双通道荧光探针
DOI：
10.1002/cjoc.201600923
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发表时间：
2017-08-01
影响因子：
5.4
通讯作者：
Guo, Yuan
中科院分区：
文献类型：
--
作者：
Pan, Shengnan;Tang, Haoyang;Guo, Yuan
设计并合成了一种新型的香豆素Schiff碱荧光探针7-羟基香豆素-3-羧酸乙酯-8-甲醛苯甲酰腙（EBH），在DMF-H_2 O（9：1，V/V）溶液中基于C = N异构化、光诱导电子转移（PET）抑制和螯合增强荧光（CHEF）机理识别Ca ~（2+），在H_2 O-CH_3OH（9：1，V/V）溶液中基于激发态分子内质子转移（ESIPT）和CHEF过程检测Zn ~（2+）。探针EBH的结构已通过单晶X射线衍射分析证实。同时，将该探针用于MCF-7细胞内Zn 2+离子的成像，获得了良好的成像效果。
A novel coumarin Schiff base fluorescent probe ethyl 7-hydroxycoumarin-3-carboxylate-8-formaldehyde benzoyl hydrazone (EBH) has been designed and synthesized which shows solvent dependent dual sensing, viz., recognition of Ca2+ in DMF-H2O (9:1, V/V) solution based on C = N isomerization, photoinduced electron transfer (PET) inhibition and chelation-enhanced fluorescence (CHEF) mechanism as well as detection of Zn2+ in H2O-CH3OH (9:1, V/V) solution by excited-state intramolecular proton transfer (ESIPT) and CHEF processes. The structure of the probe EBH has been confirmed by single-crystal X-ray diffraction analysis. Meanwhile, the probe was also used to image intracellular Zn2+ ions in MCF-7 cells with a good performance.