Isolation of botulinolysin, a thiol-activated hemolysin, from serotype D Clostridium botulinum: A species-specific gene duplication in Clostridia
Isolation of botulinolysin, a thiol-activated hemolysin, from serotype D Clostridium botulinum: A species-specific gene duplication in Clostridia
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从血清型 D 肉毒杆菌中分离肉毒杆菌溶血素（一种硫醇激活的溶血素）：梭状芽胞杆菌中的物种特异性基因重复
DOI：
10.1016/j.micres.2016.08.011
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发表时间：
2016
影响因子：
6.7
通讯作者：
and Toshihiro Watanabe
中科院分区：
文献类型：
--
作者：
Tomonori Suzuki;Thomas Nagano;Koichi Niwa;Shingo Mutoh;Masataka Uchino;Motohiro Tomizawa;Yoshimasa Sagane;and Toshihiro Watanabe
肉毒杆菌溶血素是一种由肉毒杆菌产生的毒素，属于硫醇激活的溶血素。在本研究中，从c的培养上清中纯化出一种具有溶血活性的蛋白。肉毒杆菌D型菌株4947。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析，该蛋白为单条带，分子量为55 kDa，其n端和内部氨基酸序列与革兰氏阳性菌产生的一组硫醇活化溶血素具有较高的相似性。因此，纯化的蛋白被鉴定为BLY。利用先前克隆的溶血素基因的核苷酸序列，意外地克隆了两种编码bly样蛋白的基因。分子模型分析表明，两个基因的产物结构非常相似，尽管序列相似性较低。在硅筛选显示一个特定的重复溶血素基因仅限于血清型C和D ofC。硫醇激活溶血素产生菌中的肉毒杆菌及其相关菌种。我们的发现为了解致病菌的遗传特征提供了重要的见解。
Botulinolysin (BLY) is a toxin produced byClostridium botulinumthat belongs to a group of thiol-activated hemolysins. In this study, a protein exhibiting hemolytic activity was purified from the culture supernatant ofC. botulinumserotype D strain 4947. The purified protein displayed a single band by sodium dodecyl sulfate polyacrylamide gel electrophoresis with a molecular mass of 55 kDa, and its N-terminal and internal amino acid sequences exhibited high similarity to a group of thiol-activated hemolysins produced by gram-positive bacteria. Thus, the purified protein was identified as the BLY. Using the nucleotide sequences of previously cloned genes for hemolysins, two types of genes encoding BLY-like proteins were cloned unexpectedly. Molecular modeling analysis indicated that the products of both genes displayed very similar structures, despite the low sequence similarity.In silicoscreening revealed a specific duplication of the hemolysin gene restricted to serotypes C and D ofC. botulinumand their related species among thiol-activated hemolysin-producing bacteria. Our findings provide important insights into the genetic characteristics of pathogenic bacteria.