Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro.
Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro.
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DOI：
10.1016/j.stemcr.2017.12.013
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发表时间：
2018-02-13
影响因子：
5.9
通讯作者：
Akazawa C
中科院分区：
文献类型：
--
作者：
Ishii K;Sakurai H;Suzuki N;Mabuchi Y;Sekiya I;Sekiguchi K;Akazawa C
卫星细胞在成熟骨骼肌的动态平衡和再生中起着前体细胞的作用。在健康组织中，这些细胞由肌纤维和基底膜形成的微环境维持在静止状态，其中层粘连蛋白(LMS)是主要成分。在本研究中，我们评估了体内卫星细胞的微环境，发现这些细胞被LMα2-5包裹。我们试图通过在重组的LM-E8片段存在的情况下体外培养卫星细胞来概括这种卫星细胞生态位。我们发现，LM-E8通过减少JNK和p38的磷酸化，在未分化状态下促进卫星细胞的增殖。在移植到损伤的肌肉组织中，用LM-E8培养的卫星细胞促进了骨骼肌的再生。这些发现表明，通过使用重组的LM-E8片段重塑卫星细胞生态位，培养卫星细胞用于移植是一种有效的方法。Lmα2-5包裹卫星细胞在未分化状态下，Lm-E8促进卫星细胞的增殖促进骨骼肌再生本研究通过体内卫星细胞微环境检测发现，这些细胞被Lmα2-5包裹，我们发现重组的Lm-E8片段重组卫星细胞生态位促进了卫星细胞的增殖，并维持了卫星细胞的未分化状态。
Satellite cells function as precursor cells in mature skeletal muscle homeostasis and regeneration. In healthy tissue, these cells are maintained in a state of quiescence by a microenvironment formed by myofibers and basement membrane in which LAMININs (LMs) form a major component. In the present study, we evaluated the satellite cell microenvironment in vivo and found that these cells are encapsulated by LMα2–5. We sought to recapitulate this satellite cell niche in vitro by culturing satellite cells in the presence of recombinant LM-E8 fragments. We show that treatment with LM-E8 promotes proliferation of satellite cells in an undifferentiated state, through reduced phosphorylation of JNK and p38. On transplantation into injured muscle tissue, satellite cells cultured with LM-E8 promoted the regeneration of skeletal muscle. These findings represent an efficient method of culturing satellite cells for use in transplantation through the recapitulation of the satellite cell niche using recombinant LM-E8 fragments. Satellite cells are encapsulated by LMα2–5 LM-E8 promotes proliferation of satellite cells in an undifferentiated state Satellite cells cultured with LM-E8 enhanced the regeneration of skeletal muscle In this study, examination of the satellite cell microenvironment in vivo revealed that these cells are encapsulated by LMα2–5. We find that reconstitution of the satellite cell niche with recombinant LM-E8 fragments promotes the proliferation and maintains satellite cells in an undifferentiated state.
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影响因子：
11.8
作者：
Conboy, IM;Rando, TA
通讯作者：
Rando, TA
影响因子：
12.4
作者：
Ikemoto, Madoka;Fukada, So-ichiro;Takeda, Shin'ichi
通讯作者：
Takeda, Shin'ichi
影响因子：
23.9
作者：
Bentzinger, C. Florian;Wang, Yu Xin;von Maltzahn, Julia;Soleimani, Vahab D.;Yin, Hang;Rudnicki, Michael A.;Rudnicki, A.
通讯作者：
Rudnicki, A.
体内扩张和体内自我更新人类肌肉干细胞。
影响因子：
5.9
作者：
Charville GW;Cheung TH;Yoo B;Santos PJ;Lee GK;Shrager JB;Rando TA
通讯作者：
Rando TA
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM