Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis
Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis
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DOI：
10.1016/s0885-5765(03)00092-4
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
Nicholson, P
中科院分区：
文献类型：
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作者：
Chandler, EA;Simpson, DR;Nicholson, P
禾谷镰刀菌、小麦镰刀菌和谷类镰刀菌是引起小麦赤霉病的主要病原，在全球范围内均有发生。这些真菌产生毛霉菌酮，主要是雪腐镰刀菌烯醇(NIV)和脱氧雪腐镰刀菌烯醇(DON)。来自毛霉烯生物合成基因簇的基因Tri13和Tri7将DON转化为NIV(Tri13)，将NIV转化为4-乙酰-NIV(Tri7)。我们建立了基于这两个基因的阳性-阴性聚合酶链式反应分析方法，准确地指示了禾谷镰刀菌、禾谷镰刀菌和禾谷镰刀菌的DON或NIV化学型。这些检测方法可用于评估三氯乙烯污染的风险，并可在流行病学研究中提供信息。所有研究的NIV化学型菌株都有Tri13和Tri7的功能拷贝，所有产生DON的菌株都有这两个基因的中断或缺失。我们已经确定了这些基因中的几个突变，这些突变在禾谷镰刀菌谱系、RAPD和SCAR组中以及在这三个物种之间都是保守的。似乎有证据表明，在毛霉烯生物合成基因簇中存在物种间的杂交。(C)2003爱思唯尔有限公司。保留所有权利。
Fusarium graminearum, Fusarium culmorum and Fusarium cerealis are major causal agents of Fusarium Head Blight (scab) which is a disease of global significance in all cereal growing areas. These fungi produce trichothecene mycotoxims, principally nivalenol (NIV) and deoxynivalenol (DON). Genes Tri13 and Tri7 from the trichothecene biosynthetic gene cluster convert DON to NIV (Tri13) and NIV to 4-acetyl-NIV (Tri7). We have developed positive-negative PCR assays based on these two genes, which accurately indicate a DON or NIV chemotype in F. graminearum, F. culmorum and F. cerealis. These assays are useful in assessing the risk of trichothecene contamination, and can be informative in epidemiological studies. All NIV chemotype isolates studied have functional copies of both Tri13 and Tri7, and all DON-producing isolates have both genes disrupted or deleted. We have identified several mutations in these genes, which are conserved across F. graminearum lineage, RAPD and SCAR groupings and between the three species. There appears to be evidence of inter-species hybridisation within the trichothecene biosynthetic gene cluster. (C) 2003 Elsevier Ltd. All rights reserved.