Inward and outward rectifying potassium currents set membrane potentials in activated rat microglia
Inward and outward rectifying potassium currents set membrane potentials in activated rat microglia
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DOI：
10.1016/s0304-3940(99)00053-1
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发表时间：
1999-03-05
影响因子：
2.5
通讯作者：
Lee, MY
中科院分区：
文献类型：
--
作者：
Chung, S;Jung, W;Lee, MY
用脂多糖（LPS）激活培养的大鼠小胶质细胞，诱导除已有的内向整流K+电流（K- ir）外的向外整流K+ (Kv)电流。通过使用全细胞膜片钳法测量零电流膜电位，我们发现K- v电流在将膜电位设置为-45 mV附近起直接作用。由于小胶质细胞的膜电位在-45 mV和-70 mV处有两个突出的峰，我们假设K-IR电流可能使膜电位在-70 mV附近。我们观察到，较大K-IR电流的细胞在-70 mV左右具有零电流膜电位，而Ba2+去极化膜电位阻断K-IR电流至-45 mV附近。这些结果表明，K-IR和K-V电流的大小决定了lps激活的小胶质细胞的零电流膜电位。1999爱思唯尔科学爱尔兰有限公司版权所有。
Activation of cultured rat microglial cells with lipopolysaccharide (LPS), induced outward rectifying K+ (Kv) current in addition to already existing inward rectifying K+ current (K-IR) BY measuring zero-current membrane-potentials using whole-cell patch-clamp method, we showed that K-V current plays a direct role in setting membrane potential to near -45 mV. Since the membrane potentials of microglia show two prominent peaks at -45 and -70 mV, we hypothesize that K-IR current might set the membrane potential to near -70 mV. We observed that cells with larger K-IR current had a zero-current membrane-potential at around -70 mV, and that blocking of K-IR current with Ba2+ depolarized membrane potentials to near -45 mV. These results indicate that the amounts of K-IR, and K-V current determine the zero-current membrane-potentials in LPS-activated microglia. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.