Substantia nigra compacta neurons that innervate the reticular thalamic nucleus in the rat also project to striatum or globus pallidus: Implications for abnormal motor behavior
Substantia nigra compacta neurons that innervate the reticular thalamic nucleus in the rat also project to striatum or globus pallidus: Implications for abnormal motor behavior
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DOI：
10.1016/j.neuroscience.2006.08.033
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发表时间：
2006-12-01
期刊：
影响因子：
3.3
通讯作者：
Erlij, D.
中科院分区：
文献类型：
--
作者：
Anaya-Martinez, V.;Martinez-Marcos, A.;Erlij, D.
通过测量 6-羟基多巴胺 (6-OHDA) 诱导的单侧病变大鼠的多巴胺含量和计数酪氨酸羟化酶阳性 (TN (+)) 细胞，以及在无病变的大鼠中使用荧光束追踪技术，评估黑质致密部 (SNc) 到网状丘脑核 (RTn) 的投射。在 RTn 中注射 6-OHDA 可使 SNc 中的多巴胺含量和 TH (+) 细胞数量减少约 50%。沉积在 RTn 中的 6-OHDA 显着减少纹状体和苍白球中的多巴胺这一发现表明 SNc 存在分支。此外，将 6-OHDA 注射到纹状体或苍白球中可显着降低 RTn 中的多巴胺含量。将荧光示踪剂注射到 SNc 中 RTn 标记的 TH (+) 细胞中。当示踪剂也注射到苍白球或纹状体中时，这些 TH (+) 细胞的很大一部分被双重标记。其他实验表明，全身注射阿扑吗啡或甲基苯丙胺可诱导 RTn 或所研究的基底神经节核中局部沉积有 6-OHDA 的大鼠的转向行为。广泛的多巴胺能分支表明，RTn 中有 6-OHDA 沉积的大鼠的异常运动行为可能是由不止一种结构的多巴胺能去神经引起的。单个多巴胺能神经元的损伤可以减少多个结构中的多巴胺传递，这一事实可能对于产生帕金森病的表现很重要。 (c) 2006 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The projections of the substantia nigra pars compacta (SNc) to the reticular thalamic nucleus (RTn) were assessed by measuring dopamine content and counting tyrosine hydroxylase positive (TN (+)) cells in rats with unilateral lesions induced by 6-hydroxydopamine (6-OHDA), and by using a fluorescent tract-tracing technique in rats without lesions. Injection of 6-OHDA in the RTn reduced dopamine content and the number of TH (+) cells in the SNc by about 50%. Branching of SNc was suggested by the finding that 6-OHDA deposited in the RTn significantly reduced dopamine in the striatum and globus pallidus. Moreover, injections of 6-OHDA into either the striatum or the globus pallidus significantly reduced dopamine content in the RTn. Fluorescent tracers injected into the RTn labeled TH (+) cells in the SNc. A high proportion of these TH (+) cells was double labeled when tracers were also injected into either the globus pallidus or striatum. Other experiments showed that systemic injection of apomorphine or methamphetamine induced turning behavior in rats with local deposits of 6-OHDA in either the RTn or the studied basal ganglia nuclei. The extensive dopaminergic branching suggests that the abnormal motor behavior of rats with 6-OHDA deposits in the RTn may be caused by dopaminergic denervation of more than one structure. The fact that lesion of a single dopaminergic neuron can reduce dopamine transmission in more than one structure is probably important in generating the manifestations of Parkinson's disease. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.