DEVELOPMENTAL-CHANGES IN METHYLATION OF SPERMATOGENESIS-SPECIFIC GENES INCLUDE REPROGRAMMING IN THE EPIDIDYMIS
DEVELOPMENTAL-CHANGES IN METHYLATION OF SPERMATOGENESIS-SPECIFIC GENES INCLUDE REPROGRAMMING IN THE EPIDIDYMIS
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DOI：
10.1038/ng0594-59
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发表时间：
1994-05-01
期刊：
影响因子：
30.8
通讯作者：
MCCARREY, J
中科院分区：
文献类型：
--
作者：
ARIEL, M;CEDAR, H;MCCARREY, J
我们已经确定了在特定位点的DNA甲基化的状态，在三个精子发生特异性基因，Pgk-2，ApoA 1和Oct-3/4，在整个小鼠雄性生殖细胞的发育和分化。我们观察到一个特定的去甲基化事件在Pgk-2基因在精原细胞在出生时，约10天前开始转录，首先发生在初级精母细胞。这三个基因在成年睾丸生精细胞中均未甲基化，但在输精管成熟精子中均重新甲基化。令人惊讶的是，我们发现这种再甲基化是发生在附睾中的精子成熟过程的一部分。
We have determined the status of DNA methylation at specific sites in three spermatogenesis-specific genes, Pgk-2, ApoA1 and Oct-3/4, throughout the development and differentiation of male germ cells in the mouse. We observed a specific demethylation event in the Pgk-2 gene in prospermatogonia at about the time of birth, about 10 days before the onset of transcription which first occurs in primary spermatocytes. All three genes were unmethylated in adult spermatogenic cells in the testis, but were remethylated in mature spermatozoa in the vas deferens. Surprisingly, we found that this remethylation is part of the process of sperm maturation which occurs in the epididymis.