Quantitative evaluation of orofacial motor function in mice: The pasta gnawing test, a voluntary and stress-free behavior test
Quantitative evaluation of orofacial motor function in mice: The pasta gnawing test, a voluntary and stress-free behavior test
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DOI：
10.1016/j.jneumeth.2016.10.006
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发表时间：
2016-12-01
影响因子：
3
通讯作者：
Hutter-Paier, B.
中科院分区：
文献类型：
--
作者：
Rabl, R.;Horvath, A.;Hutter-Paier, B.
背景资料：啮齿类动物运动缺陷的评估主要局限于肢体运动测试，这些测试通常是动物的高压力源。新方法：为了在无压力环境中测试啮齿类动物的口面运动障碍，我们通过测量吃意大利面的小鼠的咀嚼噪音来建立意大利面咀嚼测试。评价了两个参数，即咬合速度和每次咬合的咬合峰值。为了评估这个测试的功率相比，常用的肢体运动和肌肉力量的测试，帕金森氏病，肌萎缩侧索硬化症和尼曼-皮克病的三个小鼠模型进行了测试，在面食啃测试，RotaRod和钢丝悬挂test.Results：我们的研究结果表明，面食啃测试可靠地显示orofacial运动缺陷。该测试作为早发性疾病模型中的附加运动测试特别有用，因为它比RotaRod或钢丝悬挂测试更晚显示首次缺陷。该测试依赖于一个自愿的饮食行为的动物，只有一个短时间的食物剥夺，因此应该是stress-free.Conclusions：啃意大利面的测试是一个有价值的工具来分析orofacial运动缺陷在不同的早发性疾病模型。(C)2016作者（S）由Elsevier B. V.出版，这是CC BY-NC-ND许可下的开放获取文章。
Background: Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals.New method: To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piece of spaghetti. Two parameters were evaluated, the biting speed and the biting peaks per biting episode. To evaluate the power of this test compared to commonly used limb motor and muscle strength tests, three mouse models of Parkinson's disease, amyotrophic lateral sclerosis and Niemann-Pick disease were tested in the pasta gnawing test, RotaRod and wire suspension test.Results: Our results show that the pasta gnawing test reliably displays orofacial motor deficits.Comparison with existing methods: The test is especially useful as additional motor test in early onset disease models, since it shows first deficits later than the RotaRod or wire suspension test. The test depends on a voluntary eating behavior of the animal with only a short-time food deprivation and should thus be stress-free.Conclusions: The pasta gnawing test represents a valuable tool to analyze orofacial motor deficits in different early onset disease models. (C) 2016 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.