Comparison of the effects of retinoids and glucocorticosteroid on protein and type IV collagen synthesis in HT-1080 (human basement membrane forming fibrosarcoma) cells.
Comparison of the effects of retinoids and glucocorticosteroid on protein and type IV collagen synthesis in HT-1080 (human basement membrane forming fibrosarcoma) cells.
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类维生素A和糖皮质激素对HT-1080（人基底膜形成纤维肉瘤）细胞中蛋白质和IV型胶原合成的影响的比较。
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
A. Oikarinen
中科院分区：
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作者：
A. Oikarinen
研究了不同维甲酸和地塞米松对人基底膜形成纤维肉瘤（HT-1080）细胞蛋白质和IV型胶原合成的影响。10（-7）M浓度的视黄醇、依托维甲酸酯（Ro-10-9359）、依托维甲酸酯游离酸（Ro-10-1670）和13-顺式维甲酸（RA）略微降低HT-1080细胞中的总蛋白和IV型胶原合成，Ro-10-1670和13-顺式维甲酸的降幅最大。与此相反，地塞米松显着刺激[14 C]脯氨酸的掺入和[14 C]羟脯氨酸的合成，IV型胶原蛋白的合成的指数。地塞米松后总蛋白质合成和IV型胶原合成的部分增加是由于脯氨酸的细胞内活性增强。维甲酸对脯氨酸比活力无明显影响。此外，13-顺式-RA与地塞米松也增加总蛋白和IV型胶原蛋白的合成，但在较小程度上比单独地塞米松。13-cis-RA不影响纤维连接蛋白的合成，结缔组织基质的一个组成部分，而地塞米松明显增加纤维连接蛋白的绝对和相对合成。因此，结果表明，类维生素A调节HT-1080细胞的代谢，在某种程度上是独立的糖皮质激素。在临床实践中体内使用的类维生素A也可能调节基底膜的结缔组织基质（即IV型胶原蛋白）的代谢。
The effects of various retinoids and dexamethasone on protein and type IV collagen synthesis were studied in human basement membrane-forming fibrosarcoma (HT-1080) cells. Retinol, etretinate (Ro-10-9359), free acid of etretinate (Ro-10-1670) and 13-cis-retinoic acid (RA) in 10(-7) M concentrations slightly reduced total protein and type IV collagen synthesis in the HT-1080 cells, the largest decrease being found with Ro-10-1670 and 13-cis-RA. In contrast, dexamethasone markedly stimulated the incorporation of [14C]proline and the synthesis of [14C]hydroxyproline, an index of the synthesis of type IV collagen. Part of the increase noted in total protein synthesis and type IV collagen synthesis after dexamethasone was due to enhanced intracellular activity of proline. Retinoids did not markedly affect the specific activity of proline. The addition of 13-cis-RA with dexamethasone also increased total protein and type IV collagen synthesis, but to a lesser extent than did dexamethasone alone. 13-cis-RA did not affect the synthesis of fibronectin, a component of connective tissue matrix, while dexamethasone clearly increased the absolute and relative synthesis of fibronectin. Thus the results indicate that retinoids modulate the metabolism of HT-1080 cells, in a way which is separate from that of glucocorticoids. It is also possible that retinoids used in vivo in clinical practice may modulate the metabolism of the connective tissue matrix of basement membranes, i.e. type IV collagen.