Studies in chemical ionization mass spectrometry: 17‐hydroxy steroids
Studies in chemical ionization mass spectrometry: 17‐hydroxy steroids
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化学电离质谱研究：17-羟基类固醇
DOI：
10.1002/oms.1210060708
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发表时间：
1972
影响因子：
2.3
通讯作者：
B. Munson
中科院分区：
文献类型：
--
作者：
J. Michnowicz;B. Munson
以甲烷为反应气体，获得了几种羟基甾体化合物的化学电离质谱。的光谱是复杂得多，比电子电离光谱和小碎片的类固醇核观察。主要碎片离子涉及[M + H]+中水的损失。甾体中的3-酮基的特征在于丰富的[M + C2 H5]+离子。5α-和5β-二氢睾酮可以通过它们的光谱来区分，以H2作为反应气体，通过[M + H]+、[M + H − H2O]+和[M + H − 2 H2O]+的量的显著差异来区分。还研究了取代的3α-X-，17 β-醇化合物（X = Cl，Br），以获得在这些位点上质子化的相对量。
The chemical ionization mass spectra of several hydroxy steroids were obtained using methane as the reactant gas. The spectra are much less complex than the electron ionization spectra and little fragmentation of the steroid nucleus is observed. The major fragment ions involve the loss of water from [M + H]+. A 3-keto group in the steroids was characterized by an abundant [M + C2H5]+ ion. 5α- and 5β-Dihydrotestosterone could be distinguished by their spectra, with H2 as the reactant gas by marked differences in amounts of [M + H]+, [M + H − H2O]+ and [M + H − 2H2O]+. Substituted 3α-X-, 17 β-ol compounds, (X = Cl, Br) were also studied to obtain relative amounts of protonation at these sites.