Bombyx mori Akirin hijacks a viral peptide vSP27 encoded by BmCPV circRNA and activates the ROS-NF-κB pathway against viral infection
Bombyx mori Akirin hijacks a viral peptide vSP27 encoded by BmCPV circRNA and activates the ROS-NF-κB pathway against viral infection
复制标题
家蚕 Akirin 劫持 BmCPV circRNA 编码的病毒肽 vSP27 并激活 ROS-NF-κB 通路对抗病毒感染
DOI：
10.1016/j.ijbiomac.2021.11.201
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发表时间：
2022
影响因子：
8.2
通讯作者：
Chengliang Gong
中科院分区：
文献类型：
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作者：
Yunshan Zhang;Xing Zhang;Kun Dai;Min Zhu;Zi Liang;Jun Pan;Ziyao Zhang;Renyu Xue;Guangli Cao;Xiaolong Hu;Chengliang Gong
家蚕轮状病毒是呼肠孤病毒科的成员之一，是轮状病毒的模式病毒，具有10个节段的双链RNA基因组。然而，到目前为止，只发现了一个对病毒感染具有负调控作用的病毒小肽vSP27；宿主与BmCPV相互作用的机制尚不清楚。在这里，我们确定了vSP27是从BmCPV衍生的环状RNA(CircRNA-vSP27)翻译而来的。结果表明，vSP27诱导ROS的产生，激活了NF-κB信号通路，诱导了抗菌肽的表达，抑制了BmCPV的感染。另一方面，我们发现了一种核蛋白Akirin，它可以劫持vSP27，正向调节NF-κB途径，从而抑制病毒感染。综上所述，我们的数据提示BmCPV来源的CircRNA-vSP27具有小肽翻译活性，可能被宿主免疫用来防御BmCPV感染。
Bombyx moricypovirus (BmCPV), a member of the family Reoviridae, is a model of Cypovirus, has a 10 segmented double-stranded RNA genome. However, so far, only one viral small peptide vSP27 with negative regulation on viral infection was identified; the mechanisms underlying host-BmCPV interaction are still unknown. Here, we identified that vSP27 was translated from a BmCPV derived circular RNA (circRNA-vSP27). Subsequently, results showed that vSP27 induced generation of ROS activated the NF-κB signaling pathway, induced the expression of antimicrobial peptides, and suppressed BmCPV infection. On the other hand, we identified a nuclear protein Akirin that could hijack vSP27, positively regulate the NF-κB pathway, and lead to inhibiting the viral infection. Altogether, our data suggested that BmCPV derived circRNA-vSP27 with small peptide translation activity may be employed by the host immunity in defense against the BmCPV infection.