Loss of function of Oryza sativa Argonaute 18 induces male sterility and reduction in phased small RNAs
Loss of function of Oryza sativa Argonaute 18 induces male sterility and reduction in phased small RNAs
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DOI：
10.1007/s00497-020-00386-w
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发表时间：
2020-03-01
影响因子：
3.4
通讯作者：
Shivaprasad, P. V.
中科院分区：
文献类型：
--
作者：
Das, Soumita;Swetha, Chenna;Shivaprasad, P. V.
关键信息在这篇手稿中，我们表明，Oryza sativa indica Argonaute 蛋白 AGO18 是雄配子体发育所必需的，可能是通过一种小 RNA 介导的机制。由于 Dicer-like 和 Argonaute (AGO) 家族成员的独特成员的存在，单子叶植物进化出了独特的基因沉默途径。在单子叶植物AGO同系物中，AGO18占有独特的地位。之前的报道表明这种蛋白与病毒抗性以及配子发生有关，可能是通过它与 AGO1 进化枝成员竞争 micro(mi)RNA 和其他小 (s)RNA 来实现的。尽管水稻 AGO18 在雄性配子发生特定阶段的表达已被记录，但其在植物发育中的主要功能仍知之甚少。在这里，我们表明 Oryza sativa indica AGO18 参与雄性配子体的发育。转基因水稻品系中 AGO18 的敲低 (KD) 会导致雄性不育的植株发育不良，而其心皮却具有功能。转录组分析显示 KD 系中几个花粉发育相关基因的下调。 KD 品系营养和生殖组织中的 sRNA 测序表明，与雄性配子体发育有关的 miRNA 和阶段性次级 sRNA 减少。我们的结果表明水稻 AGO18 在男性生育力中发挥着独特的作用。
Key message In this manuscript, we show that Oryza sativa indica Argonaute protein AGO18 is required for male gametophyte development likely to through a small RNA-mediated mechanism. Monocots have evolved unique gene silencing pathways due to the presence of unique members of Dicer-like and Argonaute (AGO) family members. Among the monocot AGO homologs, AGO18 occupies a unique position. Previous reports have implicated this protein in viral resistance as well as in gametogenesis, likely through its competition with AGO1 clade members for micro(mi)RNAs and other small (s)RNAs. Although expression of rice AGO18 in specific stages of male gametogenesis has been documented, its major functions in plant development remain poorly understood. Here, we show that Oryza sativa indica AGO18 is involved in male gametophyte development. Knockdown (KD) of AGO18 in transgenic rice lines resulted in stunted plants that are male sterile, whereas their carpels were functional. Transcriptome analysis revealed downregulation of several pollen development-associated genes in KD lines. sRNA sequencing in vegetative and reproductive tissues of KD lines indicated reduction of miRNAs and phased secondary sRNAs implicated in male gametophyte development. Our results indicate a distinct role for rice AGO18 in male fertility.