Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19
Arabidopsis histone deacetylase HDA9 regulates flowering time through repression of AGL19
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DOI：
10.1016/j.bbrc.2012.11.102
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发表时间：
2013-03-08
影响因子：
3.1
通讯作者：
Zhou, Dao-Xiu
中科院分区：
文献类型：
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作者：
Kim, Wanhui;Latrasse, David;Zhou, Dao-Xiu
开花时间受光周期、春化等多种调控途径的严格控制，其中包括表观遗传过程。在这项工作中，我们发现拟南芥组蛋白去乙酰化酶基因HDA9参与开花时间控制。该基因的突变导致短日照植物的早期开花表型，而长日照植物则没有影响。花期调控基因表达分析表明，hda9突变可高度诱导AGL19的表达，但对CO、SOC1和FLC无影响。染色质免疫沉淀试验表明，突变导致AGL19基因上的组蛋白H3K9和H3K27乙酰化在短时间内明显增加。AGL19以独立于CO和PLC途径的方式促进开花，并被polycomb group suppressicomplex 2 (PRC2) EMF2抑制，但被春化激活。hda9突变诱导的AGL19表达和组蛋白乙酰化水平与长日照条件下的水平相当，表明AGL19也受日照调节，hda9通过促进组蛋白H3去乙酰化参与AGL19的短日照抑制，这可能与PRC2 EMF2复合物有关。(C) 2012爱思唯尔公司版权所有。
Flowering time is tightly controlled by several regulatory pathways including photoperiod, vernalization in which epigenetic processes are involved. In this work, we have found that the Arabidopsis histone deacetylase gene HDA9 is involved in flowering time control. Mutation of the gene led to an early flowering phenotype in short day grown plants while without effect in long days. Analysis of flowering time regulatory gene expression revealed that hda9 mutations highly induced the expression of AGL19, but had no effect on CO, SOC1 or FLC. Chromatin immunoprecipitation assays indicated that the mutations led to a clear increase of histone H3K9 and H3K27 acetylation on the AGL19 gene in short days. AGL19 promotes flowering in a way independent of the CO and PLC pathways and has been shown to be repressed by polycomb group repressive complex2 (PRC2) EMF2 but activated by vernalization. The induced levels of AGL19 expression and histone acetylation by the hda9 mutations were comparable to that of the gene under long day conditions, indicating that AGL19 is regulated also by day length and that HDA9 is involved in short day repression of AGL19 by promoting histone H3 deacetylation, which may be related to the PRC2 EMF2 complex. (C) 2012 Elsevier Inc. All rights reserved.