Identification of c-di-GMP/FleQ-regulated new target genes,including cyaA, encoding adenylate cyclase, in Pseudomonas putida
Identification of c-di-GMP/FleQ-regulated new target genes,including cyaA, encoding adenylate cyclase, in Pseudomonas putida
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DOI：
10.1128/msystems.00295-21
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发表时间：
2021
期刊：
影响因子：
6.4
通讯作者：
Qiaoyun Huanga
中科院分区：
文献类型：
--
作者：
Yujie Xiao;Haozhe Chen;Liang Nie;Meina He;Qi Peng;Wenjing Zhu;Hailing Nie;Wenli Chen;Qiaoyun Huanga
The bacterial second messenger cyclic diguanylate (c-di-GMP) modulates plankton-to-biofilm lifestyle transition of Pseudomonas species through its transcriptional regulatory effector FleQ. FleQ regulates transcription of biofilm- and flagellar-related genes in response to c-di-GMP. Through transcriptomic analysis and FleQ-DNA binding assay, this study identified five new target genes of c-di-GMP/FleQ in P. putida including PP_0681, PP_0788, PP_4519 (lapE), PP_5222 (cyaA), and PP_5586. Except lapE encoding an outer membrane pore protein and cyaA encoding an adenylate cyclase, the functions of other three genes encoding hypothetical proteins remain unknown. FleQ and c-di-GMP coordinately inhibit transcription of PP_0788 and cyaA, and promote transcription of PP_0681, lapE, and PP_5586. Both in vitro and in vivo assays show that FleQ binds directly to promoters of the five genes. Further analyses confirm that LapE plays a central role of in the secretion of adhesin LapA, and that c-di-GMP/FleQ increases lapE transcription, thus promoting adhesin secretion and biofilm formation. The adenylate cyclase CyaA is responsible for synthesis of another second messenger cAMP. FleQ and c-di-GMP coordinate to decrease the content of cAMP, suggesting that c-di-GMP and FleQ co-regulate cAMP by modulating cyaA expression. Overall, this study adds five new members to the c-di-GMP/FleQ-regulated gene family, and reveals the role of c-di-GMP/FleQ in LapA secretion and cAMP synthesis regulation in P. putida.
