Dietary choline requirement for juvenile cobia, Rachycentron canadum
Dietary choline requirement for juvenile cobia, Rachycentron canadum
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DOI：
10.1016/j.aquaculture.2009.01.016
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发表时间：
2009-04-03
期刊：
影响因子：
4.5
通讯作者：
Ren, Mingchun
中科院分区：
文献类型：
--
作者：
Mai, Kangsen;Xiao, Lindong;Ren, Mingchun
进行了10wk饲养试验，以确定幼鱼对胆碱的需要量。基础日粮的粗蛋白含量为47.1 g，干重为100g(-1)，由无维生素的酪蛋白、明胶和鱼蛋白浓缩而成。这种预混料提供了1.05%的蛋氨酸。略低于豚鱼的最适需要量(1.19%)，限制了蛋氨酸内源合成胆碱。在基础日粮中添加氯化胆碱，配制出6种精制日粮，分别含133、350、548、940、2017和3981 mg·kg~(-1)胆碱。每种饲料随机饲喂三组，初始平均体重4.2+/-0.4g的幼鱼在流动系统中饲养。饲料中胆碱水平显著影响豚鼠的存活率、摄食率、增重、饲料效率、肝体指数以及肝脏和肌肉中胆碱的含量。体重增加、肝脏和肌肉胆碱浓度的折线回归分别得出696、877和950 mg胆碱kg(-1)饮食中以氯化胆碱形式存在的胆碱需要量。此外，饲料中添加胆碱显著提高了军曹鱼的肌脂含量。补充胆碱对肌肉脂肪及相关风味和质地的潜在影响值得进一步研究。(C)2009年，爱思唯尔出版。
A 10-wk feeding trial was conducted to determine dietary choline requirement for juvenile cobia. The basal diet was formulated to contain 47.1 g crude protein 100 g(-1) dry weight from vitamin-free casein, gelatin and fish protein concentrate. This premix provided methionine at 1.05%. slightly less than the optimal requirement of cobia (1.19%), so endogenous synthesis of choline from methionine would be limited. Choline chloride was supplemented to the basal diet to formulate six purified diets containing 133 (control group), 350, 548, 940, 2017 and 3981 mg choline kg(-1) diet, respectively. Each diet was randomly fed to triplicate groups of juvenile cobia with initial average weight 4.2 +/- 0.4 g in a flow-through system. Dietary choline level significantly influenced survival, feeding rate, weight gain, feed efficiency ratio, hepatosomatic index, as well as the choline concentrations in the liver and muscle of cobia. Broke-line regression of weight gain, liver and muscle choline concentration yield choline requirements of 696, 877 and 950 mg choline kg(-1) diet in the form of choline chloride, respectively. In addition, dietary choline supplementation significantly increased muscle lipid content of cobia. Potential manipulation of muscle lipid and associated flavor and texture by choline supplementation warrants further investigation. (C) 2009 Published by Elsevier B.V.