Purification of hydroxyanthraquinones and cinnamic acid from the roots of Rheum officinale Baill
Purification of hydroxyanthraquinones and cinnamic acid from the roots of Rheum officinale Baill
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DOI：
10.1002/jssc.200700430
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Liu, Renmin
中科院分区：
文献类型：
--
作者：
Huang, Yanyan;Sun, Ailing;Liu, Renmin
本文报道了中药大黄化学成分的分离纯化方法。通过使用12%交联琼脂糖凝胶Superose 12作为分离介质建立。采用两步分离程序。以65%甲醇为洗脱剂，从大黄粗提物中分离肉桂酸、大黄酸、大黄素甲醚和大黄素。然后用含0.5%乙酸的55%甲醇作为洗脱剂分离芦荟大黄素和大黄酚。结果得到肉桂酸和大黄酸、芦荟大黄素、大黄酚、大黄素甲醚和大黄素等5种羟基蒽醌类化合物。研究了羟基蒽醌类化合物在Superose 12上的保留行为。羟基蒽醌在Superose 12上的保留是基于羟基蒽醌的羟基与Superose 12生产工艺中使用的交联剂残基之间的氢键和疏水相互作用的混合物。
A chromatographic method for isolation and purification of chemical constituents from the well-known traditional Chinese drug Da-huang (roots of Rheum officinale Baill.) was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. A two-step separation procedure is employed. Sixty five percent methanol was used as the eluent for separation of cinnamic acid, rhein, physcion and emodin form Da-huang crude extract. The fraction containing aloe-emodin and chrysophanol was then separated by using 55% methanol containing 0.5% acetic acid as the eluent. As a result, cinnamic acid and five kinds of hydroxyanthraquinones including rhein, aloe-emodin, chrysophanol, physcion and emodin were obtained. The retention behavior of hydroxyanthraquinones on Superose 12 was also studied. The retention of hydroxyanthraquinones on Superose 12 is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of the hydroxyanthraquinones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.