Established and emerging fluorescence-based assays for G-protein function: Ras-superfamily GTPases.
Established and emerging fluorescence-based assays for G-protein function: Ras-superfamily GTPases.
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DOI：
10.2174/138620703106298509
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发表时间：
2003-05
影响因子：
1.8
通讯作者：
Rafael J. Rojas;R. Kimple;K. Rossman;D. Siderovski;J. Sondek
中科院分区：
文献类型：
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作者：
Rafael J. Rojas;R. Kimple;K. Rossman;D. Siderovski;J. Sondek
Ras和Rho GTP酶是调节多种生理事件的信号蛋白，并且与癌症的进展密切相关。最近，各种基于荧光的测定已经被改进以监测这些GTP酶的激活。本文综述了目前基于荧光的Ras超家族GTP酶的研究技术，重点是实际的例子和高通量的应用。这些技术不仅可用于Ras超家族成员的生物化学表征，而且还将促进设计用于抑制由GTP酶介导的信号转导的小分子治疗剂的发现。
Ras and Rho GTPases are signaling proteins that regulate a variety of physiological events and are intimately linked to the progression of cancer. Recently, a variety of fluorescence-based assays have been refined to monitor activation of these GTPases. This review summarizes current fluorescence-based techniques for studying Ras superfamily GTPases with an emphasis on practical examples and high-throughput applications. These techniques are not only useful for biochemical characterization of Ras superfamily members, but will also facilitate the discovery of small molecule therapeutics designed to inhibit signal transduction mediated by GTPases.