Differential effect of steady versus oscillating flow on bone cells
Differential effect of steady versus oscillating flow on bone cells
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DOI：
10.1016/s0021-9290(98)00114-6
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发表时间：
1998-11-01
影响因子：
2.4
通讯作者：
Donahue, HJ
中科院分区：
文献类型：
--
作者：
Jacobs, CR;Yellowley, CE;Donahue, HJ
最近，负载诱导的流体流动被认为是骨力转导中的重要生物物理信号。由对骨骼施加负荷的活动（例如站立、运动或姿势肌肉活动）产生的流体流动预计本质上是动态的，并且包括相对较小的静态分量。然而，迄今为止，骨细胞的体外流体流动实验仅使用稳定或脉冲流动曲线进行。在这项研究中，我们将成骨细胞样 hFOB 1.19 细胞（永生化人胎儿成骨细胞）暴露于精确控制的补充有 2% 胎牛血清的盐水动态流体流动曲线中，同时用荧光染料 fura-2 监测细胞内钙浓度。应用的流包括导致 2 N m(-2) 壁剪切应力的稳定流、振荡流 (+/- 2 N m(-2)) 和脉冲：流（0 至 2 N m(-2)）。动态流动采用 0.5、1.0 和 2.0 Hz 的正弦曲线。我们发现，与稳定流或脉冲流相比，振荡流对骨细胞的刺激作用要弱得多。此外，观察到动态 Hows 的响应能力随着频率的增加而降低。在这两种情况下，响应性的降低与流动剖面的净流体传输的降低同时发生。因此，这些发现支持这样的假设：骨细胞对液体流动的反应依赖于化学转运效应。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
Loading induced fluid flow has recently been proposed as an important biophysical signal in bone mechanotransduction. Fluid flow resulting from activities which load the skeleton such as standing, locomotion, or postural muscle activity are predicted to be dynamic in nature and include a relatively small static component. However, in vitro fluid flow experiments with bone cells to date have been conducted using steady or pulsing How profiles only. In this study we exposed osteoblast-like hFOB 1.19 cells (immortalized human fetal osteoblasts) to precisely controlled dynamic fluid flow profiles of saline supplemented with 2% fetal bovine serum while monitoring intracellular calcium concentration with the fluorescent dye fura-2. Applied Hows included steady How resulting in a wall shear stress of 2 N m(-2), oscillating flow (+/- 2 N m(-2)), and pulsing: flow (0 to 2 N m(-2)). The dynamic flows were applied with sinusoidal profiles of 0.5, 1.0, and 2.0 Hz. We found that oscillating flow was a much less potent stimulator of bone cells than either steady or pulsing How. Furthermore, a decrease in responsiveness with increasing frequency was observed for the dynamic Hows. In both cases a reduction in responsiveness coincides with a reduction in the net fluid transport of the flow profile. Thus, these findings support the hypothesis that the response of bone cells to fluid flow is dependent on chemotransport effects. (C) 1998 Elsevier Science Ltd. All rights reserved.