Electrode calibration with a microfluidic flow cell for fast-scan cyclic voltammetry.
Electrode calibration with a microfluidic flow cell for fast-scan cyclic voltammetry.
复制标题
使用微流体流动池进行电极校准，用于快速扫描循环伏安法。
DOI：
10.1039/c2lc40168a
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发表时间：
2012
期刊：
影响因子：
6.1
通讯作者：
Eddington,DavidT
中科院分区：
文献类型：
--
作者：
Sinkala,Elly;McCutcheon,JamesE;Schuck,MatthewJ;Schmidt,Eric;Roitman,MitchellF;Eddington,DavidT
快速扫描循环伏安法（FSCV）是一种常用的分析电化学工具，用于测量化学物种。它最近被用于测量清醒和行为动物（体内）的神经递质，如多巴胺。电极校准是FSCV中将观察到的电流与化学物质的浓度相关联的重要步骤。然而，现有的方法需要多个组件，这降低了校准的容易性。为此，开发了一种微流体流动池（μFC），作为一种简单的装置，用于在微流体Y通道中在缓冲液和具有已知浓度的目标分析物（在这种情况下为多巴胺）的缓冲液之间切换。快速切换溶液的能力产生了具有更快上升时间的电极校准，并且在峰值电流值下更稳定。μFC减少了外部电子元件的数量，并在一定浓度范围内进行线性校准。为了证明这一点，使用μFC校准的电极用于大鼠在食物奖励期间的FSCV记录-由于多巴胺的氧化，这种刺激可靠地引起电流的短暂增加。使用线性校准，从行为任务期间诱发的电流响应确定多巴胺浓度。μFC能够轻松快速地校准FSCV电极对体外和体内实验中化学物质的响应。
Fast-scan cyclic voltammetry (FSCV) is a common analytical electrochemistry tool used to measure chemical species. It has recently been adapted for measurement of neurotransmitters such as dopamine in awake and behaving animals (in vivo). Electrode calibration is an essential step in FSCV to relate observed current to concentration of a chemical species. However, existing methods require multiple components, which reduce the ease of calibrations. To this end, a microfluidic flow cell (μFC) was developed as a simple device to switch between buffer and buffer with a known concentration of the analyte of interest – in this case dopamine – in a microfluidic Y-channel. The ability to quickly switch solutions yielded electrode calibrations with faster rise times and that were more stable at peak current values. The μFC reduced the number of external electrical components and produced linear calibrations over a range of concentrations. To demonstrate this, an electrode calibrated with the μFC was used in FSCV recordings from a rat during the delivery of food reward – a stimulus that reliably evokes a brief increase in current due to the oxidation of dopamine. Using the linear calibration, dopamine concentrations were determined from the current responses evoked during the behavioral task. The μFC is able to easily and quickly calibrate FSCV electrode responses to chemical species for both in vitro and in vivo experiments.