Ouabain‐induced increases in resting tone of human hyperplastic prostate following repeated noradrenaline and electrical field stimulation
Ouabain‐induced increases in resting tone of human hyperplastic prostate following repeated noradrenaline and electrical field stimulation
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重复去甲肾上腺素和电场刺激后哇巴因诱导人增生性前列腺静息张力增加
DOI：
10.1111/j.1476-5381.1996.tb15344.x
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发表时间：
1996
影响因子：
7.3
通讯作者：
C. Teng
中科院分区：
文献类型：
--
作者：
J. Guh;F. Ko;S. Chueh;M. Lai;C. Teng
1观察哇巴因对去甲肾上腺素重复刺激和电场刺激引起的人前列腺增生症收缩的影响。哇巴因(1μm)本身不能引起人前列腺收缩反应，但递增人前列腺的静息张力(即一次去甲肾上腺素刺激或电场刺激之间的张力)。2反复刺激去甲肾上腺素或电场刺激后哇巴因引起的张力增加可通过去除外源性钙而完全松弛，恢复钙后即可恢复。3测定去甲肾上腺素对Na+摄取的影响。去甲肾上腺素(10μm)显著增加哇巴因(1μm)引起的Na+蓄积，这种作用可被哌唑嗪(0.1μm)和乙基异丙基阿米洛利(10 0μm)几乎完全阻断。河豚毒素(1μm)对去甲肾上腺素刺激的人前列腺细胞Na+转运无影响。4去甲肾上腺素(10μm)加哇巴因(1μm)可显著增加细胞跨膜钙摄取，但硝苯地平(1μm)对此无明显影响。5去甲肾上腺素(10μm)可刺激人前列腺细胞跨膜Ca~(2+)外流，哇巴因(1μm)可明显抑制这种作用。结果提示，哇巴因抑制Na+泵，使Na+/Ca+交换系统的Ca~(2+)内流增加和Ca~(2+)外流减少，从而使人前列腺增生症的张力增加。
1 The effect of ouabain on contractions to repeated noradrenaline stimulation and electrical field stimulation of human hyperplastic prostate was examined. Ouabain (1 μm) did not induce contractile response per se but progressively increased the resting tone (i.e., the tone between one noradrenaline stimulation, or electrical field stimulation, and the following) of human hyperplastic prostate. 2 The increased tone by ouabain following repeated noradrenaline stimulations or electrical field stimulation was fully relaxed by the removal of external calcium, and recovered following restoration of calcium. 3 The effect of noradrenaline on Na+ uptake was measured. Noradrenaline (10 μm) significantly increased the rate of Na+ accumulation in the presence of ouabain (1 μm); this stimulatory effect was almost completely blocked by prazosin (0.1 μm) and ethylisopropylamiloride (100 μm). In contrast, tetrodotoxin (1 μm) had no effect on noradrenaline‐stimulated Na+ transport in human hyperplastic prostate. 4 Intracellular Na+ loading by noradrenaline (10 μm) in the presence of ouabain (1 μm) significantly increased the transmembrane Ca2+ uptake as compared with the absence of ouabain; however, nifedipine (1 μm) was ineffective on Ca2+ uptake under this condition. 5 Transmembrane Ca2+ efflux was stimulated by noradrenaline (10 μm) in human hyperplastic prostate; this effect was significantly decreased in the presence of ouabain (1 μm). 6 It is suggested that the increased tone of human hyperplastic prostate following repeated excitation in the presence of ouabain is due to increased Ca2+ entry and reduced efflux of Ca2+ through the Na+/Ca+ exchange system as a consequence of Na+ pump inhibition by ouabain.
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DOI：
10.1210/jcem-58-1-76
发表时间：
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期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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作者：
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影响因子：
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DOI：
10.1152/ajpcell.1984.246.5.c422
发表时间：
1984
期刊：
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影响因子：
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DOI：
10.1152/ajpcell.1988.254.1.c75
发表时间：
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期刊：
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影响因子：
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