THE RESPONSE OF ANTHER CULTURE IN A GENETICALLY WIDE MATERIAL OF WINTER-WHEAT (TRITICUM-AESTIVUM L)
THE RESPONSE OF ANTHER CULTURE IN A GENETICALLY WIDE MATERIAL OF WINTER-WHEAT (TRITICUM-AESTIVUM L)
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DOI：
10.1111/j.1439-0523.1987.tb01170.x
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发表时间：
1987-11-01
期刊：
影响因子：
2
通讯作者：
OLESEN, A
中科院分区：
文献类型：
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作者：
ANDERSEN, SB;DUE, IK;OLESEN, A
对215个冬小麦品种进行了筛选试验。对于花药培养的反应，从200个不同的品种和93个不同的品种的绿色植株中获得花粉胚或愈伤组织。平均每100个花药培养得到1.3个绿色植株。方差分量估计与选定部分的植物材料的重复表明，从花药花粉胚的形成，基因型和复制之间的相互作用占主导地位，占45%至50%的变化。来自基因型的主效应不太突出，占变异的25%至30%，而重复的影响相对较小，占总变异的4%至12%。从花粉胚或愈伤组织再生植株的基因型或复制没有显着影响。再生植株的频率是绿色，然而，基因型和环境的影响，分别贡献总变异的42.5%和34.9%，而只有轻微的基因型和环境之间的相互作用表明，这个字符。
In a screening experiment with 215 different winter cultivars of Triticum aestivum L. for response in anther culture, pollen embryos or callus were obtained from 200 different cultivars and green plants from 93 different cultivars. On average, from the whole material, 1.3 green plants were obtained per 100 anthers cultured. Variance components estimated from replicates with selected parts of the plant material indicated that for the formation of pollen embryos from anthers, interactions between genotypes and replications were dominating, accounting for 45 to 50 per cent of the variation. Main effects from genotypes were less prominent, accounting for 25 to 30 per cent of the variation, and the replications showed relatively little effect, accounting for about 4 to 12 per cent of total variation. Regeneration of plants from pollen embryos or callus was not significantly influenced by either genotype or replicates. The frequencies of plants regenerated being green, however, were influenced from both genotype and environment contributing 42.5 and 34.9 per cent of the total variation respectively, while only minor interactions between genotype and environments were indicated for this character.