MITOCHONDRIAL MEMBRANE POTENTIAL, MORPHOLOGY AND ATP PRODUCTION IN MAMMALIAN CELLS EXPOSED TO X-RAYS
MITOCHONDRIAL MEMBRANE POTENTIAL, MORPHOLOGY AND ATP PRODUCTION IN MAMMALIAN CELLS EXPOSED TO X-RAYS
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暴露于 X 射线的哺乳动物细胞中的线粒体膜电位、形态和 ATP 生成
DOI：
10.1093/rpd/ncy254
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发表时间：
2019
影响因子：
1
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Hamada;R. Kaminaga;K. Suzuki;K. Yokoya;A.
本研究旨在从线粒体膜电位(ΔΨm)和生产力方面揭示X射线照射对线粒体功能的影响。在细胞水平上，使用JC-1对ΔΨm进行定量，JC-1是一种线粒体探针，分别在ΔΨm的高或低位点发出红色或绿色荧光。将两种颜色的荧光面积一起相对于同一细胞的整个细胞面积进行量化。不同受照细胞的荧光面积与全细胞面积的差异很大，这取决于所接受的X射线剂量(6和10GY)和孵育时间，尽管相对红色面积与总线粒体面积的关系是相当恒定的。照射后48h，大部分G1期滞留细胞的平均ATP浓度暂时升高，并达到最大值。这些结果表明，每个细胞线粒体体积的增加，而不仅仅是其活性部位的增加，导致了ATP产量的增加。
This study aimed to reveal the effect of X-irradiation on mitochondrial function in terms of mitochondrial membrane potential (ΔΨm) and ATP productivity. At the cellular level, ΔΨm was quantified using JC-1, a mitochondrial probe that emits red or green fluorescence at high or low ΔΨm sites, respectively. The fluorescence area was quantified for both colors together relative to the whole-cell area of the same cell. The fluorescence areas versus whole-cell areas varied widely among the irradiated cells depending on the X-ray doses received (6 and 10 Gy) and incubation time, although the relative red area to total mitochondrial area was rather constant. Average ATP concentrations temporarily increased and showed a maximum at 48 h after irradiation for largely G1-arrested cells. These results indicate that an increase of mitochondrial volume per cell, not simply an increase in their active sites, is induced by irradiation, resulting in enhanced ATP production.