The Regulation of Muscle Structure and Metabolism by Mio/dChREBP in Drosophila.
The Regulation of Muscle Structure and Metabolism by Mio/dChREBP in Drosophila.
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DOI：
10.1371/journal.pone.0136504
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
DiAngelo JR
中科院分区：
文献类型：
--
作者：
Polak GL;Pasqualino A;Docherty JE;Beck SJ;DiAngelo JR
所有细胞都需要能量来执行其特殊功能。由于肌肉收缩需要高能量，肌肉对营养的可用性特别敏感。因此，肌肉细胞获取、储存和利用能量的能力对于这些细胞的功能至关重要。 Mio 是果蝇碳水化合物反应元件结合蛋白 (ChREBP) 的同源物，最近被确定为营养反应转录因子，对于果蝇脂肪体内甘油三酯的储存非常重要。然而，Mio 在肌肉中的功能尚不清楚。在这项研究中，我们表征了 Mio 在控制肌肉功能和新陈代谢中的作用。使用 RNAi 降低肌肉中的 Mio 水平会导致胸腔糖原储存增加。电子显微镜显示，由于间接飞行肌肉中肌原纤维形状和大小的改变，成年 Mio-RNAi 果蝇也存在飞行缺陷。果蝇在从蛹中出来之前，肌原纤维的大小也会减小，这表明 Mio 在肌原纤维发育中发挥着作用。总之，这些数据表明 Mio 在控制肌肉结构和新陈代谢方面发挥着新作用，并可能提供营养可用性和肌肉功能之间的分子联系。
All cells require energy to perform their specialized functions. Muscle is particularly sensitive to the availability of nutrients due to the high-energy requirement for muscle contraction. Therefore the ability of muscle cells to obtain, store and utilize energy is essential for the function of these cells. Mio, the Drosophila homolog of carbohydrate response element binding protein (ChREBP), has recently been identified as a nutrient responsive transcription factor important for triglyceride storage in the fly fat body. However, the function of Mio in muscle is unknown. In this study, we characterized the role of Mio in controlling muscle function and metabolism. Decreasing Mio levels using RNAi specifically in muscle results in increased thorax glycogen storage. Adult Mio-RNAi flies also have a flight defect due to altered myofibril shape and size in the indirect flight muscles as shown by electron microscopy. Myofibril size is also decreased in flies just before emerging from their pupal cases, suggesting a role for Mio in myofibril development. Together, these data indicate a novel role for Mio in controlling muscle structure and metabolism and may provide a molecular link between nutrient availability and muscle function.