Inflammatory signals in the development of tissue-engineered soft tissue.
Inflammatory signals in the development of tissue-engineered soft tissue.
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DOI：
10.1016/j.biomaterials.2007.08.004
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发表时间：
2007-12
期刊：
影响因子：
14
通讯作者：
N. Rhodes
中科院分区：
文献类型：
--
作者：
N. Rhodes
将具有400μm孔的支架（由羧酸基团的苄基酯化改性的透明质酸（HYAFF-11）和由透明质酸的十二烷基酰胺化（HYADD-3）产生的粘性凝胶构建）皮下植入大鼠体内，分别长达26周和12周。用甲基丙烯酸酯树脂浸润组织外植体，切片，并除常规组织学染色外，还用广泛的炎症标志物组染色。凝胶和海绵都被细胞迅速浸润，在HYAFF海绵的情况下，细胞没有分化，而成熟的脂肪细胞仅在海绵的边缘观察到。这与持续的炎症抗原表达相结合。相反，在HYADD凝胶中，在4周时仅观察到中度炎症染色，其在8周时完全减少。在凝胶深处观察到成熟和正在成熟的脂肪细胞。据推测，凝胶呈现出优异的炎性细胞因子谱，其诱导巨噬细胞浸润、增殖，然后分化成脂肪细胞，并负责新脂肪形成的产生。
Scaffolds with 400μm pores constructed from hyaluronan modified by benzyl esterification of the carboxylic acid groups (HYAFF-11) and viscous gels created from dodecyl-amidation of hyaluronan (HYADD-3) were implanted subcutaneously into rats for periods of up to 26 and 12 weeks, respectively. Tissue explants were infiltrated with methacrylate resin, sectioned and stained with a broad panel of inflammatory markers in addition to conventional histological stains. Both gels and sponges became rapidly infiltrated by cells that, in the case of HYAFF sponges, did not differentiate, whilst mature adipocytes were only observed at the margins of the sponges. This was combined with sustained inflammatory antigen expression. Conversely, in the HYADD gels, only moderate inflammatory staining was observed at 4 weeks which had diminished completely by 8 weeks. Mature and maturing adipocytes were observed deep within the gels. It is hypothesised that the gels present an excellent inflammatory cytokine profile which induces macrophage infiltration, proliferation then differentiation into adipocytes and is responsible for the generation of neoadipogenesis.