Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis
Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis
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DOI：
10.1073/pnas.96.2.715
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发表时间：
1999-01-19
影响因子：
11.1
通讯作者：
Ausubel, FM
中科院分区：
文献类型：
--
作者：
Tan, MW;Mahajan-Miklos, S;Ausubel, FM
我们发现，一个单一的临床分离的人类机会致病菌铜绿假单胞菌（菌株PA 14），这以前被证明是致病的小鼠和植物，也杀死秀丽隐杆线虫，PA 14介导的杀死率的C。线虫的生长取决于PA 14生长的琼脂培养基的组成。当PA14在基本培养基上生长时，杀死发生在数天的过程中，并且被称为“缓慢”杀死。当PA 14在高渗透压介质中呻吟时，杀死发生在几个小时的过程中，被称为“快速”杀死。几条证据，包括热杀死的细菌仍然能够快速而不是缓慢地杀死C。elegans的研究表明，快速和缓慢的杀伤通过不同的机制发生，缓慢的杀伤涉及感染样过程，并与PA 14在蠕虫肠道内的积累相关。在10个与PA14毒力相关的突变体中，有6个对C.在快速和缓慢杀伤条件下对秀丽隐杆线虫的感染，表明铜绿假单胞菌在不同的真核宿主中致病所需的因子之间具有高度的共性。从宿主和病原体的角度来看在遗传上易于处理的线虫致病性模型可用于模拟哺乳动物细菌致病性。
We show that a single clinical isolate of the human opportunistic pathogen Pseudomonas aeruginosa (strain PA14),which previously was shown to be pathogenic in mice and plants, also kills Caenorhabditis elegans, The rate of PA14-mediated killing of C. elegans depends on the composition of the agar medium on which PA14 is grown. When PA14 is grown on minimal medium, killing occurs over the course of several days and is referred to as "slow" killing. When PA14 is groan on high-osmolarity medium, killing occurs over the course of several hours and is referred to as "fast" killing. Several lines of evidence, including the fact that heat-killed bacteria are still capable of fast but not slow killing of C. elegans, indicate that fast and slow killing occur by distinct mechanisms, Slow killing involves an infection-like process and correlates with the accumulation of PA14 within worm intestines. Among 10 PA14 virulence-related mutants that had been shown previously to affect pathogenicity in plants and mice, 6 were less effective in killing C. elegans under both fast- and slow-killing conditions, indicating a high degree of commonalty among the P, aeruginosa factors required for pathogenicity in disparate eukaryotic hosts, Thus, we show that a C. elegans pathogenicity model that is genetically tractable from the perspectives of both host and pathogen can be used to model mammalian bacterial pathogenesis.