Involvement of Polycomb-group genes in establishing HoxD temporal colinearity
Involvement of Polycomb-group genes in establishing HoxD temporal colinearity
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DOI：
10.1002/dvg.20326
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发表时间：
2007-09-01
期刊：
影响因子：
1.5
通讯作者：
Kondo, Takashi
中科院分区：
文献类型：
--
作者：
Mishra, Rakesh K.;Yamagishi, Takumi;Kondo, Takashi
小鼠HoxD中的时间共线性依赖于复合物5'端内序列的抑制活性。我们发现，从这个区域的5-kb的DNA片段抑制转基因时，结合在小鼠以及在果蝇此外，在果蝇的抑制活性依赖于一些成员的Polycomb组（PcG）基因，例如，额外的性梳。我们还表明，这些因素与抑制片段的直接关联，无论是在转基因苍蝇和本地小鼠HoxD复合物的背景下。这些结果表明，在两个非常不同的物种和一些PcG基因的HoxD复杂的全球抑制区域的功能参与建立早期的抑制状态的HoxD复杂的，从而有助于时间共线性。
Temporal colinearity in mouse HoxD is dependent on repressive activity of sequences within the 5' end of the complex. We show that a 5-kb DNA fragment from this region represses transgenes when combined in mouse as well as in Drosophila melanogaster Moreover, repressive activity in Drosophila depends on some members of the Polycomb-group (PcG) genes, for example, extra sex combs. We also showed direct association of these factors with the repressive fragment, both in transgenic flies and in the context of the native mouse HoxD complex. These results suggest that the global repressive region of the HoxD complex functions in two very different species and that some PcG genes are involved in establishing the early repressive state of the HoxD complex, thus contributing to temporal colinearity.