Transcriptional behavior of EUL-related rice lectins toward important abiotic and biotic stresses
Transcriptional behavior of EUL-related rice lectins toward important abiotic and biotic stresses
复制标题
DOI：
10.1016/j.jplph.2014.04.004
复制
发表时间：
2014-07-15
影响因子：
4.3
通讯作者：
Van Damme, Els J. M.
中科院分区：
文献类型：
--
作者：
Al Atalah, Bassam;De Vleesschauwer, David;Van Damme, Els J. M.
水稻基因组编码几个推定的糖结合蛋白基因，属于卫矛属相关凝集素家族。该凝集素家族是最近发现的，证据表明这些蛋白的表达受多种环境胁迫的影响。在本研究中，对暴露于各种非生物（150 mM NaCl、100 mM甘露醇和100 μ M脱落酸（阿坝））和生物（Xanthomonas oryzae pv.稻瘟病菌和稻瘟病菌）胁迫以比较EUL和属于jacalin-相关凝集素家族的已知胁迫相关凝集素Orysata的转录行为。阿坝和NaCl处理后，根中所有EUL转录物均强烈上调，而茎中的总体表达水平通常较低且更易变化。此外，除甘露醇处理对根没有影响外，所有非生物胁迫均在两种组织中诱导了Orysata。Orysata在X. oryzaepv.稻的感染，以及各种D-型EUL凝集素。与此相反，一些EUL蛋白，包括OrysaEULS 3、OrysaEULD 1A和OrysaEULD 2，以及Orysata在M.稻的攻击，这表明真菌操纵了这些基因。总之，我们的结果清楚地表明，水稻表达多种碳水化合物结合蛋白，以响应各种各样的非生物和生物胁迫条件。我们推测，卫矛属植物的相关蛋白在感知和响应多种环境信号方面发挥着重要作用。(C)2014爱思唯尔有限公司All rights reserved.
The rice genome encodes several genes for putative carbohydrate-binding proteins belonging to the family of Euonymus related lectins (EULs). This lectin family was discovered recently and evidence shows that the expression of these proteins is subject to multiple environmental stresses. In this study, quantitative reverse transcription PCR (qRT-PCR) was conducted on rice seedlings exposed to various abiotic (150 mM NaCl, 100 mM mannitol, and 100 mu M abscisic acid (ABA)) and biotic (Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae) stresses to compare the transcriptional behavior of the EULs and a known stress related lectin Orysata belonging to the family of jacalin-related lectins. All EUL transcripts were strongly up-regulated after ABA and NaCl treatments in the roots whereas the overall expression level was generally lower and more variable in the shoots. Moreover, all abiotic stresses induced Orysata in both tissues except for mannitol treatment which failed to show an effect in the roots. Orysata also strongly accumulated after X. oryzae pv. oryzae infection, as were various D-type EUL lectins. In contrast, some of the EUL proteins, including OrysaEULS3, OrysaEULD1A and OrysaEULD2, as well as Orysata were significantly down-regulated upon M. oryzae attack, suggesting fungal manipulation of these genes. Collectively, our results clearly show that rice expresses multiple carbohydrate-binding proteins in response to a wide variety of abiotic and biotic stress conditions. We hypothesize that the Euonymus related proteins fulfill a prominent role in sensing and responding to multiple environmental cues. (C) 2014 Elsevier GmbH. All rights reserved.